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. Author manuscript; available in PMC: 2021 Jul 15.
Published in final edited form as: J Comp Neurol. 2020 Jan 24;528(11):1833–1855. doi: 10.1002/cne.24857

Table 3.

Quantification of EGFPVgat-labeled TH-immunoreactive neurons from Vgat-cre;L10-Egfp mouse brain tissue

Brain region1 Abbrev.2 ARA Level3 Number of neurons4
TH-ir EGFPVgat + TH-ir5 Coloc.6 (%)
Periventricular zone
Supraoptic nucleus SO 56–63 07 0
Accessory supraoptic group ASO n/a8 n/a
Paraventricular hypothalamic nucleus
    Magnocellular division
    Anterior magnocellular part  PVHam  n/a  n/a
    Medial magnocellular part  PVHmm 60–65  2 ± 1  1 ± 1
    Posterior magnocellular part
       Lateral zone PVHpml 60–65 0 0
       Medial zone PVHpmm 63 0 0
    Parvicellular division
       Anterior parvicellular part PVHap 56–59 37 ± 5 33 ± 6 90
       Dorsal zone PVHmpd 60–66 5 ± 2 3 ± 2 63
       Periventricular part PVHpv 56–65 3 ± 2 2 ± 1 60
Periventricular hypothalamic nucleus
    Anterior part PVa 59–61 8 ± 4 6 ± 4 90
    Intermediate part PVi9 62–64 7 ± 1 6 ± 1 91
65–67 27 ± 4 23 ± 3 87
68–74 4 ± 2 3 ± 1 84
Arcuate hypothalamic nucleus ARH9 65–67 5 ± 3 4 ± 3 73
68–71 85 ± 18 74 ± 16 87
72–74 27 ± 9 23 ± 9 88
Periventricular region
Anteroventral preoptic nucleus ADP 51–53 0 0
Anterior hypothalamic area AHA n/a n/a
Anteroventral preoptic nucleus AVP 50–54 1 ± 1 1 ± 1
Anteroventral periventricular nucleus AVPV 51–53 5 ± 4 1 ± 0 13
Dorsomedial nucleus of the hypothalamus
  Anterior part DMHa9 67–69 29 ± 8 27 ± 8 92
70–74 5 ± 3 4 ± 2 93
  Posterior part DMHp 70–74 11 ± 3 9 ± 3 81
  Ventral part DMHv 71–74 10 ± 2 9 ± 2 87
Median preoptic nucleus MEPO n/a n/a
Medial preoptic area MPO9 50–55 9 ± 5 9 ± 4 98
56–58 4 ± 3 3 ± 2 73
Vascular organ of the lamina terminalis OV n/a n/a
Posterodorsal preoptic nucleus PD 0 0
Parastrial nucleus PS 0 0
Suprachiasmatic preoptic nucleus PSCH n/a n/a
Periventricular hypothalamic nucleus
    Posterior part PVp 75–79 8 ± 2 5 ± 2 72
    Preoptic part PVpo 54–66 54 ± 4 50 ± 5 93
Subparaventricular zone SBPV 60–63 32 ± 1 30 ± 0 93
Subfornical organ SFO n/a n/a
Ventrolateral preoptic nucleus VLPO 53–55 0 0
Hypothalamic medial zone
Anterior hypothalamic nucleus
  Anterior part  AHNa 58–64 0 0
  Central part  AHNc 59–64 1 ± 1 1 ± 0
  Dorsal part  AHNd n/a n/a
  Posterior part AHNp 63–66 6 ± 1 6 ± 1 88
Mammillary body
  Lateral mammillary nucleus LM 78–81 0 0
  Medial mammillary nucleus MM 79–82 0 0
  Supramammillary nucleus
    Lateral part SUMl 78–82 4 ± 2 0 0
    Medial part SUMm 78–82 6 ± 6 0 0
  Tuberomammillary nucleus
    Dorsal part TMd 75–77 0 0
    Ventral part TMv 77–79 0 0
Medial preoptic nucleus
  Central part MPNc 53–55 0 0
  Lateral part  MPNl 54–58 1 ± 0 1 ± 0
  Medial part  MPNm 54–58 3 ± 1 1 ± 0 57
Dorsal premammillary nucleus PMd
Ventral premammillary nucleus PMv
Paraventricular hypothalamic nucleus, descending
  Dorsal parvicellular part PVHdp 60–65 0 0
  Forniceal part PVHf n/a n/a
  Lateral parvicellular part PVHlp 66 4 ± 2 4 ± 2 95
  Medial parvicellular part, ventral PVHmpv 64–65 0 0
Ventromedial hypothalamic nucleus
  Anterior part VMHa 65–66 0 0
  Central part VMHc 67–73 1 ± 1 1 ± 1
  Dorsomedial part VMHdm 67–71 0 0
  Ventrolateral part VMHvl 66–73 0 0
Hypothalamic lateral zone
Lateral hypothalamic area LHA 59–79 15 ± 11 6 ± 5 37
Lateral preoptic area LPO 50–59 1 ± 1 1 ± 1
Posterior hypothalamic nucleus PH 70–82 8 ± 4 1 ± 0 12
Preparasubthalamic nucleus PST n/a n/a
Parasubthalamic nucleus PSTN 75–78 8 ± 5 2 ± 1 18
Retrochiasmatic area RCH n/a n/a
Subthalamic nucleus STN 75–78 8 ± 4 2 ± 2 30
Tuberal nucleus TU ZI9 65–75 8 ± 4 3 ± 1 43
Zona incerta 61–66 0 0
67–69 86 ± 19 86 ± 18 100
70–83 4 ± 1 2 ± 1 57
  Dopaminergic group ZIda 67–69 10 ± 5 10 ± 5 100
  Fields of Forel FF 78–79 1 ± 1 0
1

Organization of brain regions based on hierarchical structure presented by the Allen Reference Atlas (ARA; Allen Institute for Brain Science, 2011).

2

Nomenclature and atlas level based on the ARA (Dong, 2008).

3

Each brain region may span several atlas levels.

4

The total number of tyrosine hydroxylase-immunoreactive (TH-ir) neurons counted bilaterally from parcellated brain regions. Data are reported as mean ± SEM (n = 3 mice), rounded to nearest whole number. Number of neurons reported is corrected for oversampling using the Abercrombie formula P = A(M/(L+M)) (Abercrombie, 1946), where P is the corrected number of neurons counted, A is the original number of neurons counted, M is the mean tissue thickness (20 μm), L is the mean cell diameter (in μm). The cell diameter may vary between each parcellated hypothalamic region, thus a distinct Abercrombie correction factor is applied for each respective region.

5

Quantification of TH-ir neurons that coexpress native EGFP fluorescence (EGFPVgat) in TH-ir neurons.

6

Percentage of TH-ir neurons that colocalized (Coloc.) with EGFP-f indicate the proportion of GABAergic TH neurons. No value will be provided if the mean of two or less than two TH-ir neurons was counted.

7

Parcellated brain regions that did not contain a TH-ir neuron.

8

Data are not available (n/a) from brain regions that either did not emerge following Nissl-based parcellations or were not parcellated in the ARA.

9

Distribution of TH-ir neuron within this region revealed noticeable breakpoints or clustering within ARA levels.