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. 2020 Dec 25;106(4):1763–1774. doi: 10.1210/clinem/dgaa958

Figure 3.

Figure 3.

Combined immunofluorescence of HLA class I/STAT1 and PKR/VP1 in GD samples and controls. Nuclear and cytosolic STAT1 was found in thyroid cells and was colocalized with HLA class I. PKR and VP1 were also colocalized within thyroid cells. A, An example of STAT1 (green) and HLA class I (red) immunofluorescence staining in GD thyroid tissue. Arrows indicate thyroid cells with nuclear STAT1 and intracellular HLA class I. B, Control sample with negative STAT1 and HLA class I staining. C, An example of VP1 (green) and PKR (red) immunofluorescence staining in GD thyroid tissue. The insets represent higher magnification (of the area outlined by the white boxes) and shows colocalized VP1 and PKR within thyrocytes. D, Control sample with negative VP1 and PKR staining. Merged images with blue nuclear DAPI staining. All scale bars at 25 μm. DAPI, 4’,6-diamidino-2-phenylindole; GD, Graves disease; HLA, human leukocyte antigen; PKR, protein kinase R; STAT1, signal transducer and activator of transcription 1; VP1, enteroviral capsid protein 1.