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. Author manuscript; available in PMC: 2021 Jul 22.
Published in final edited form as: Science. 2021 Jan 22;371(6527):eabc3393. doi: 10.1126/science.abc3393

Fig. 3. JARID2 and AEBP2 interact with ubiquitin and the H2A-H2B surface.

Fig. 3.

(A) Cryo-EM density (contour level: 0.007) and model of JARID2 (amino acids 22 to 56) (magenta) showing how this segment of JARID2 is sandwiched between ubiquitin and the histone surface. The JARID2 UIM (amino acids 24 to 41) forms an alpha helix that interacts with both ubiquitin and nucleosomal DNA. JARID2 (amino acids 42 to 56), which contains positively charged arginine and lysine residues, sits on top of the conserved H2A-H2B acidic patch. The blue spheres with dashed lines connecting them indicate the H2AK119C-G76C conjugation. (B) The tandem C2H2 zinc fingers of AEBP2 (amino acids 50 to 116) interact with ubiquitin, the H2A-H2B surface, and nucleosomal DNA. The second AEBP2 zinc finger (amino acids 85 to 116) interacts extensively with an H2A-H2B surface that contains a mixture of positively and negatively charged residues (blue and orange balls, respectively).