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. 2021 Mar 25;16(3):e0248241. doi: 10.1371/journal.pone.0248241

Fig 3. Expression of lysosomal markers in cultures of human proximal tubule cells treated with 5.5 mM, 7.5 mM, 11 mM and 16 mM glucose for 3 passages.

Fig 3

RT-qPCR and Western blot analysis of (A) CLCN7; (B) NPC2; (C) LIPA; (D) RRAGD; (E) SQSTM1; (F) LAMP1. The top graphs in the figure represents PCR data whereas the bottom graphs represent the IOD of the Western blot analysis. ****; ***; **; * indicates significant differences in gene expression level compared to the control 5.5 mM glucose concentration at p-value of ≤ 0.0001; ≤ 0.001; ≤ 0.01; ≤ 0.05 respectively.