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. 2021 Mar 25;16(3):e0248241. doi: 10.1371/journal.pone.0248241

Fig 4. Expression of mTOR and associated genes.

Fig 4

Cultures of hRTE cells were treated with 5.5 mM, 7.5 mM, 11 mM and 16 mM glucose for 3 passages. RT-qPCR and Western blot analysis of (A) mTOR; (B) EIF4EBP1; (C) RAPTOR; (D) RICTOR. The top graphs in the figure represent the PCR data whereas the bottom graphs represent the IOD of the Western blot analysis. ****; ***; **; * indicates significant differences in gene expression level compared to the control 5.5mM glucose concentration at p-value of ≤ 0.0001; ≤ 0.001; ≤ 0.01; ≤ 0.05 respectively.