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. Author manuscript; available in PMC: 2021 Mar 25.
Published in final edited form as: Neuropharmacology. 2017 Jan 31;117:149–157. doi: 10.1016/j.neuropharm.2017.01.028

Fig. 3. Expression of aSyn in the OLN-93 stably transfected cell lines.

Fig. 3.

(A). Immunoblot of lysates of OLN-93 cell lines expressing empty vector, WT-aSyn, G51D-aSyn, or A53E-aSyn show aSyn only in cells transfected with aSyn-plasmids. (B). Confocal microscopic images of OLN-93 cell lines expressing empty vector, WT-aSyn, G51D-aSyn, or A53E-aSyn show aSyn immunoreactivity (Syn-1 antibody 1:100 dilution, red signal) only in aSyn-transfected cell lines. Stained nuclei are also shown in blue (DAPI). Scale bar = 50 μm.