Skip to main content
. Author manuscript; available in PMC: 2021 Mar 25.
Published in final edited form as: Neuropharmacology. 2017 Jan 31;117:149–157. doi: 10.1016/j.neuropharm.2017.01.028

Fig. 7. FTY720 specifically increases BDNF promoter 1 histone 3 acetylation (AcH3) in OLN-93 cells relative to controls.

Fig. 7.

ChIP-qPCR analysis (n = 3/condition) normalized as percentage of input, shows that 12 h 160 nM FTY720 significantly increases AcH3 levels at BDNF promoter 1 (BDNF-P1), but not BDNF promoter 4 (BDNF-P4), which shows a trend toward an increase as compared to vehicle treated cells. FTY720 does not change AcH3 levels at the Gapdh promoter. Control ChIP-qPCR assays using normal rabbit IgG (IgG) antibody shows only extremely low nonspecific binding for all conditions. Student’s t-test; *p < 0.05.