A-C)
In vivo
(A) proliferation and (B) renewal screens using an shRNA lentivirus pool targeting eIF2B5-regulated HrasG12V-dependent genes reveal (C) a distinct of genes that specifically inhibit renewal without affecting proliferation (red). n=3 biological replicates (DESeq2 FDR<0.05).
D) Ingenuity pathway analysis of renewal inhibitors identifies enrichment for ubiquitination genes.
E) Screen results for individual shRNAs targeting all ubiquitination genes in the screening pool. Purple shading demonstrates clustering of significantly altered shRNAs towards the pro-renewal axis.
See also Figure S4 and Table S5.