KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| chicken anti-GFP | Abcam | ab13970; RRID:AB_300798 |
| mouse anti-BrdU (MoBU-1) | Invitrogen | B35128; RRID:AB_2536432 |
| rabbit anti-Keratin 10 (Poly19054) | BioLegend | 905403; RRID:AB_2749902 |
| mouse anti-Puromycin (12D10) | Millipore | MABE343; RRID:AB_2566826 |
| guinea pig anti-Keratin 5 | Origene | BP5006 |
| CD49f/α6-Integrin-PerCP (GoH3) | BioLegend | 313617 RRID:AB_1575054 |
| CD326/EpCAM-APC (G8.8) | BD Biosciences | 563478; RRID:AB_2738234 |
| mouse anti-EIF2B5 (B-7) | Santa Cruz Biotechnology | Sc-55558; RRID:AB_831253 |
| mouse anti-β-Actin (2D4H5) | Proteintech | 66009; RRID:AB_2687938 |
| mouse anti-EIF2α (L57A5) | Cell Signaling | 2103S; RRID:AB_836874 |
| rabbit anti-phospho-EIF2α Ser51 (119A11) | Cell Signaling | 3597S; RRID:AB_390740 |
| rabbit anti-FBXO32 (EPR9148(2)) | Abcam | ab168372 |
| mouse anti-V5 (V5-10) | Sigma-Aldrich | V8012; RRID:AB_261888 |
| Bacterial and Virus Strains | ||
| TRC1.0 Lentiviral Mouse Genome shRNA Library | Sigma-Aldrich | Discontinued, now MISSION shRNA Library |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| DMEM | Gibco | 11995-065 |
| UltraCULTURE media | Lonza | 12-725F |
| E media | Nowak and Fuchs, 2009 | N/A |
| Fetal bovine serum | Gibco | 16000-044 |
| Polybrene | Sigma | 107689-10G |
| Trypsin-EDTA | GIBCO | 25200-056 |
| Chelex 100 | Bio-Rad | 1422822 |
| M.O.M. Blocking Reagent | Vector | MKB-22113 |
| ProLong Gold Antifade Mountant | ThermoFisher | P36930 |
| Antigen Unmasking Solution, Citric Acid Based | Vector | H-3300-250 |
| RIPA Lysis Buffer System | Santa Cruz Biotech | Sc-24948 |
| ISRIB | Sigma-Aldrich | SML0843 |
| SuperSignal Wester Pico PLUS Chemiluminescent Substrate | Thermo Fisher | 34577 |
| iScript Reverse Transcription Supermix | Bio-Rad | 1708840 |
| SYBR Green PCR Master Mix | Thermo Fisher | 4309155 |
| Lipofectamine 3000 | Thermo Fisher | L3000001 |
| BrdU | Invitrogen | B23151 |
| EdU | Invitrogen | A10044 |
| Puromycin | Thermo Fisher | A11138-03 |
| Cyclohexamide | Sigma-Aldrich | C7698 |
| Bortezomib | Sigma-Aldrich | 504314 |
| Dispase | Corning | 354235 |
| Mitomycin C | Sigma-Aldrich | M4287 |
| Critical Commercial Assays | ||
| Click-iT EdU Alexa Fluor 555 Imaging Kit | Invitrogen | C10338 |
| 20S Proteasome Activity Assay | Millipore | APT280 |
| PE Active Caspase-3 Apoptosis Kit | BD Biosciences | 550914 |
| DNeasy Blood & Tissue Kit | Qiagen | 69504 |
| RNeasy Plus Mini Kit | Qiagen | 74134 |
| Q5 Site-Directed Mutagenesis Kit | NEB | E0554S |
| Pierce BCA Protein Assay Kit | Thermo Fisher | 23225 |
| Dual-Luciferase Reporter Assay System | Promega | E1910 |
| TruSeq Ribo Profile (Mammalian) Library Prep Kit | Illumina | RPHMR12126 |
| Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) | Illumina | MRZH11124 |
| Deposited Data | ||
| Raw and analyzed data | This paper | GEO GSE 126660 |
| Mouse reference genome NCBI build 38 (mm10) | Genome Reference Consortium | https://www.ncbi.nlm.nih.gov/assembly/GCF_000001635.20/ |
| Experimental Models: Cell Lines | ||
| Mouse: WT primary keratinocytes | This paper | N/A |
| Mouse: HrasG12V primary keratinocytes | This paper | N/A |
| Mouse: NIH/3T3 cells | ATCC | CRL-1658; RRID:CVCL_0594 |
| Human: 293TN cells | System Biosciences | LV900A-1 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Rosa26YFPFYP | The Jackson Laboratory | 006148; RRID:IMSR_JAX:006148 |
| Mouse: Rosa26mT/mG | The Jackson Laboratory | 007676; RRID:IMSR_JAX:007676 |
| Mouse: FR-HrasG12V | Donated by James A. Fagin | N/A |
| Oligonucleotides | ||
| See Table S6 for shRNA and PCR primer sequences | ||
| Recombinant DNA | ||
| Plasmid: pLKO.1 | Addgene | 10878; RRID:Addgene_10878 |
| Plasmid: pLKO.1-Cre | Addgene | 25997; RRID:Addgene_25997 |
| Plasmid: pLX-Cre-EF1α ORF vector | Ying et al., 2018 | N/A |
| Plasmid: pLX-shRNA-Cre-EF1α ORF vector | This paper | N/A |
| Plasmid: pMD2.G | Addgene | 12259; RRID:Addgene_12259 |
| Plasmid psPAX2 | Addgene | 12260; RRID:Addgene_12260 |
| Plasmid: CMV-Luc2CP/ARE | Addgene | 62857; RRID:Addgene_62857 |
| Software and Algorithms | ||
| FASTX-Toolkit | Hannon Lab | http://hannonlab.cshl.edu/fastx_toolkit/ |
| BWA | Li et al., 2009 | http://bio-bwa.sourceforge.net/ |
| Samtools | Li et al., 2009 | http://samtools.sourceforge.net/ |
| DESeq2 | Love et al., 2014 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| Bowtie | Langmead et al., 2009 | https://bioconductor.org/packages/release/bioc/html/Rbowtie.html |
| TopHat2 | Kim et al., 2013 | https://ccb.jhu.edu/software/tophat/index.shtml |
| HTSeq | Anders et al., 2015 | https://htseq.readthedocs.io/en/release_0.10.0/ |
| Xtail | Xiao et al., 2016 | https://github.com/xryanglab/xtail |
| edgeR | Robinson et al., 2010 | https://bioconductor.org/packages/release/bioc/html/edgeR.html |
| riboseqR | Chung et al., 2015 | http://bioconductor.org/packages/release/bioc/html/riboSeqR.html |
| Ingenuity Pathway Analysis | Qiagen | https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis/ |
| Imaris | Bitplane | https://imaris.oxinst.com/ |
| ImageJ | Abramoff et al., 2004 | https://imagej.nih.gov/ij/ |
| Prism 7 | GraphPad | https://www.graphpad.com/ |
| MEME | Bailey et al., 2009 | http://meme-suite.org/tools/meme |
| FIMO | Grant et al., 2011 | http://meme-suite.org/tools/fimo |
| ZEN | Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen-lite.html |