Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background Saccharomyces cerevisiae | Yeast GFP collection co-expressing Tom70-mCherry | PMID:27097106 | N/A | N/A |
| Strain, strain background (S. cerevisiae) | BY4741 | ATCC | Cat # 201388 | N/A |
| Strain, strain background (S. cerevisiae) | BY4743 | ATCC | Cat # 201390 | N/A |
| Strain, strain background (S. cerevisiae) | Other strains used in this study | This paper | Supplementary file 2 | N/A |
| Antibody | Anti-GFP (mouse monoclonal) | Millipore Sigma | Cat # 11814460001 RRID: AB_390913 | WB (1:2500) |
| Antibody | Anti-HA (mouse monoclonal) | Millipore Sigma | Cat # 11583816001 RRID: AB_514506 |
WB (1:1000), IF (1:200) |
| Antibody | Anti-PGK1 (mouse monoclonal) | Abcam | Cat # 22C5D8 RRID: AB_2756444 |
WB (1:1000) |
| Antibody | Anti-FLAG M2 (mouse monoclonal) | Millipore Sigma | Cat # F1804 RRID: AB_262044 | IF (1:200) |
| Antibody | Anti-H2B (rabbit polyclonal) | Active Motif | Cat # 39947 RRID: AB_2793403 |
WB (1:1000) |
| Antibody | Anti-Idh1 (goat polyclonal) | ORIGENE | Cat # Ap31099PU-N | WB (1:500) |
| Antibody | Anti-Ilv2 (rabbit polyclonal) | Dr. Agnieszka Chacinska | N/A | WB (1:1000), IF (1:200) |
| Antibody | Anti-Tom70 (rabbit polyclonal) | Dr. Nikolaus Pfanner | N/A | WB (1:1000) |
| Antibody | Anti-Tom20 (rabbit polyclonal) | Dr. Nikolaus Pfanner | N/A | WB (1:1000), IF (1:200) |
| Antibody | Anti-Tim54 (rabbit polyclonal) | Dr. Nikolaus Pfanner | N/A | WB (1:1000) |
| Antibody | Anti-Tim50 (rabbit polyclonal) | Dr. Nikolaus Pfanner | N/A | WB (1:1000) |
| Antibody | Anti-Tim18 (rabbit polyclonal) | Dr. Nikolaus Pfanner | N/A | WB (1:1000) |
| Antibody | Anti-OM45 (rabbit polyclonal) | Dr. Carla Koehler | N/A | WB (1:1000), IF (1:200) |
| Antibody | anti-Tom40 (rabbit polyclonal) | Dr. Toshiya Endo | N/A | WB (1:1000) |
| Recombinant DNA reagent | pKT128 (plasmid) | Addgene | Plasmid # 8729 | N/A |
| Recombinant DNA reagent | pKT127-mCherry (plasmid) | Daniel Gottschling | N/A | N/A |
| Recombinant DNA reagent | pFA6a-5FLAG-KanMX6 (plasmid) | Addgene | Plasmid # 15983 | N/A |
| Recombinant DNA reagent | pFA6A-mCherry-HphMX (plasmid) | Addgene | Plasmid # 105156 | N/A |
| Recombinant DNA reagent | pVL015 (plasmid) | Addgene | Plasmid # 64766 | N/A |
| Recombinant DNA reagent | pFA6A-3HA-KanMX (plasmid) | Addgene | Plasmid # 39295 | N/A |
| Recombinant DNA reagent | Template plasmid for 35S-radiolabeled Su9-DHFR | Walter Neupert PMID:2884042 | N/A | N/A |
| Recombinant DNA reagent | Other plasmids generated in this study | This paper | Supplementary file 4 | N/A |
| Sequenced-based reagent | Oligos used in this study | This paper | Supplementary file 3 | N/A |
| Chemical compound, drug | -Estradiol | Millipore Sigma | Cat # E8875; CAS # 50-28-2 | N/A |
| Chemical compound, drug | Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) | Millipore Sigma | Cat # C2920; CAS # 370-86-5 | N/A |
| Chemical compound, drug | cOmplete Protease Inhibitor Cocktail | Millipore Sigma | Cat# 11697498001 | N/A |
| Chemical compound, drug | Dimethyl sulfoxide | Millipore Sigma | Cat # D2650; CAS # 67-68-5 | N/A |
| Chemical compound, drug | Cycloheximide | Millipore Sigma | Cat # C1988; CAS # 66-81-9 | N/A |
| Chemical compound, drug | Doxycycline hyclate | Millipore Sigma | Cat # C9891; CAS # 24390-14-5 | N/A |
| Chemical compound, drug | Polyvinylpyrrolidone | Millipore Sigma | Cat # PVP40; CAS # 9003-39-8 | N/A |
| Chemical compound, drug | Pepstatin | Millipore Sigma | Cat # 10253286001; CAS # 26305-03-3 | N/A |
| Chemical compound, drug | Phenylmethylsulfonyl fluoride | Millipore Sigma | Cat # P7626; CAS # 329-98-6 | N/A |
| Chemical compound, drug | Calcofluor Fluorescent Brightener 28 | Millipore Sigma | Cat # F3543; CAS # 4404-43-7 | N/A |
| Chemical compound, drug | Diamidino-2-phenylindole dihydrochloride (DAPI) | Thermo Fisher | Cat # D1306 | N/A |
| Chemical compound, drug | Glass Antifade Mountant with NucBlue Stain (P36981) | Thermo Fisher | Cat # P36981 | N/A |
| Chemical compound, drug | (S)-MG-132 | Cayman Chemical | Cat # 10012628; CAS # 133407-82-6 | N/A |
| Chemical compound, drug | N-ethylmaleimide (NEM) | Sigma–Aldrich | Cat # S3876; CAS# 128-53-0 | N/A |
| Chemical compound, drug | IGEPAL NP-40-substitute | Sigma–Aldrich | Cat # CA-630; CAS # 9002-93-1 | N/A |
| Chemical compound, drug | Zymolyase 100T | US Biological Life Sciences | Cat # Z1004; CAS# 37340-57-1 | N/A |
| Chemical compound, drug | Triton X-100 | Bio-Rad | Cat # 1610407; CAS# 9002-93-1 | N/A |
| Chemical compound, drug | Formaldehyde 16% in aqueous solution, EM Grade | VWR | Cat # 100503–914; CAS# 50-00-0 | N/A |
| Chemical compound, drug | DTT (dithiothreitol) (>99% pure) protease free | GOLDBIO | Cat # DTT10; CAS# 27565-41−9/3483- 12–3 | N/A |
| Chemical compound, drug | Antimycin A | Sigma–Aldrich | Cat # A8674; CAS# 1397-94-0 | N/A |
| Chemical compound, drug | Phusion polymerase | New England Biolabs | Cat # M0530L | N/A |
| Chemical compound, drug | Valinomycin | Sigma–Aldrich | Cat # V0627; CAS# 2001-95-8 | N/A |
| Chemical compound, drug | Oligomycin | Sigma–Aldrich | Cat # 75351; CAS# 579-13-5 | N/A |
| Commercial assay or kit | Gibson Assembly Cloning Kit | New England Biolabs | Cat # E5510S | N/A |
| Commercial assay or kit | TNT SP6 Quick Coupled Transcription/Translation System | Promega | Cat # L2080 | N/A |
| Software, Algorithm | FIJI | PMID:22743772 | Version 1 | N/A |
| Software, Algorithm | Prism | Graphpad Software | Version 8 | N/A |
| Software, Algorithm | SnapGene | GSL Biotech | Version 4.2 | N/A |
| Software, Algorithm | Image Lab | Bio-Rad | Version 6 | N/A |
| Software, Algorithm | Zen Blue | Carl Zeiss | Version 2.6 | N/A |
| Software, Algorithm | Zen Black | Carl Zeiss | Version 2.3 | N/A |
| Software, Algorithm | Mitoprot | PMID:8944766 | N/A | N/A |
| Software, Algorithm | Illustrator CC | Adobe | Version 22.1 | N/A |
| Software, Algorithm | Photoshop CC | Adobe | Version 19.1 | N/A |