Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (M. xanthus) | pomX | NCBI |
mxan_0636
new locus tag MXAN_RS03090 |
|
| Gene (M. xanthus) | pomY | NCBI |
mxan_0634
new locus tag MXAN_RS03080 |
|
| Gene (M. xanthus) | pomZ | NCBI |
mxan_0635
new locus tag MXAN_RS03085 |
|
| Strain, strain background (E. coli) | Arctic Express DE3 RP | Agilent Technologies | E. coli B F– ompT hsdS(rB– mB–) dcm+TetR gal λ(DE3) endA Hte [cpn10 cpn60 GentR] | Used for protein expression |
| Strain, strain background (E. coli) | Rosetta 2 DE3 | Novagen | F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE2 (CamR) | Used for protein expression |
| Strain, strain background (E. coli) | NiCo21(DE3) | New England Biolabs | can::CBD fhuA2 [lon] ompT gal (λ DE3) [dcm] arnA::CBD slyD::CBD glmS6Ala ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5 | Used for protein expression |
| Strain, strain background (E. coli) | NEB Turbo | New England Biolabs | F' proA+B+ lacIq(∆lacZM15/fhuA2 ∆(lac-proAB) glnV galK16 galE15 R(zgb-210::Tn10) TetS endA1 thi-1 ∆(hsdS-mcrB)5) | Used for cloning |
| Strain, strain background (M. xanthus) | DK1622 | DOI: 10.1073/pnas.76.11.5952 | Wildtype | |
| Strain, strain background (M. xanthus) | SA3108 | DOI: 10.1111/mmi.12094 | ΔpomZ | Strain with an in-frame deletion in pomZ |
| Strain, strain background (M. xanthus) | SA3146 | DOI: 10.1111/mmi.12094 | ΔpomZ; attB::Pmxan0635 pomZD90A-mCh (pKA43) | Strain expressing PomZD90A-mCh in a ΔpomZ background |
| Strain, strain background (M. xanthus) | SA4223 | https://doi.org/10.1016/j.devcel.2017.04.011 | ΔpomX | Strain with an in-frame deletion in pomX |
| Strain, strain background (M. xanthus) | SA4252 | https://doi.org/10.1016/j.devcel.2017.04.011 | ΔpomX; attB::Pmxan0635 mCh-pomX (pAH53) | Strain expressing mCh-PomX in a ΔpomX background |
| Strain, strain background (M. xanthus) | SA4703 | https://doi.org/10.1016/j.devcel.2017.04.011 | ΔpomY | Strain with an in-frame deletion in pomY |
| Strain, strain background (M. xanthus) | SA4712 | https://doi.org/10.1016/j.devcel.2017.04.011 | ΔpomY; attB::PpilA pomY-mCh (pDS7) | Strain expressing PomY-mCh in a ΔpomY background |
| Strain, strain background (M. xanthus) | SA4797 | https://doi.org/10.1016/j.devcel.2017.04.011 | ΔmglA; ΔpomX; attB::Pmxan0635 mCh-pomX (pAH53) | Strain expressing mCh-PomX in a non-motile ΔpomX background |
| Strain, strain background (M. xanthus) | SA4297 | this study | Wild-type; attB::Pmxan0635 mCh-pomX (pAH53) | Strain expressing mCh-PomX in WT background |
| Strain, strain background (M. xanthus) | SA6100 | this study | pomX::pomXK13AR15A | Strain with a replacement of pomX with the pomXK13AR15A allele |
| Strain, strain background (M. xanthus) | SA7014 | this study | ΔpomX; ΔpomZ; attB::Pmxan0635 pomZD90A-mCh (pKA43) | Strain expressing PomZD90A-mCh in a pomX and pomZ deletion background |
| Strain, strain background (M. xanthus) | SA7061 | this study | ΔmglA; ΔpomZ; ΔpomX; attB::Pmxan0635 mCh-pomX (pAH53) | Strain expressing mCh-PomX in a non-motile pomX and pomZ deletion background |
| Strain, strain background (M. xanthus) | SA7063 | this study | ΔpomZ; ΔpomX; attB::Pmxan0635 mCh-pomX (pAH53) | Strain expressing mCh-PomX in a pomX and pomZ deletion background |
| Strain, strain background (M. xanthus) | SA8240 | this study | pomX::pomXK13AR15A; ΔpomZ; attB::Pmxan0635 pomZD90A-mCh (pKA43) | Strain expressing PomZD90A-mCh in a pomZ deletion background with pomXK13AR15A mutation. |
| Strain, strain background (M. xanthus) | SA8250 | this study | pomX::pomXK13AR15A; ΔpomY; attB::PpilA pomY-mCh (pDS7) | Strain expressing PomZD90A-mCh in a pomXK13AR15A background |
| Strain, strain background (M. xanthus) | SA8268 | this study | pomX::pomXK13AR15A; ΔpomY; ΔpomZ; attB::Pmxan0635 pomZD90A-mCh (pKA43) | Strain expressing PomZD90A-mCh in a pomZ and pomY deletion background with pomXK13AR15A mutation |
| Strain, strain background (M. xanthus) | SA9700 | this study | pomX::pomXE6A | Strain with a replacement of pomX with the pomXE6A allele |
| Strain, strain background (M. xanthus) | SA9701 | this study | pomX::pomXQ7A | Strain with a replacement of pomX with the pomXQ7A allele |
| Strain, strain background (M. xanthus) | SA9702 | this study | pomX::pomXN8A | Strain with a replacement of pomX with the pomXN8A allele |
| Strain, strain background (M. xanthus) | SA9714 | this study | pomX::pomXR11A | Strain with a replacement of pomX with the pomXR11A allele |
| Strain, strain background (M. xanthus) | SA9715 | this study | pomX::pomXK3A | Strain with a replacement of pomX with the pomXK3A allele |
| Strain, strain background (M. xanthus) | SA9716 | this study | pomX::pomXR17A | Strain with a replacement of pomX with the pomXR17A allele |
| Strain, strain background (M. xanthus) | SA9717 | this study | pomX::pomXT22A | Strain with a replacement of pomX with the pomXT22A allele |
| Strain, strain background (M. xanthus) | SA9718 | this study | pomX::pomXK2A | Strain with a replacement of pomX with the pomXK2A allele |
| Strain, strain background (M. xanthus) | SA9719 | this study | pomX::pomXR15A | Strain with a replacement of pomX with the pomXR15A allele |
| Strain, strain background (M. xanthus) | SA9720 | this study | ΔpomY; ΔpomZ; attB::Pmxan0635 pomZD90A-mCh (pKA43) | Strain expressing PomZD90A-mCh in a pomZ and pomY deletion background |
| Strain, strain background (M. xanthus) | SA9721 | this study | ΔpomX; ΔpomY; attB::PpilA pomY-mCh (pDS7) | Strain expressing PomY-mCh in a pomY and pomX deletion background |
| Strain, strain background (M. xanthus) | SA9726 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXN (pDS252) | Strain expressing mCh-PomXN in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9727 | this study | Wild-type; attB::Pmxan0635 mCh-pomXN (pDS252) | Strain expressing mCh-PomXN in a WT background |
| Strain, strain background (M. xanthus) | SA9731 | this study | pomX::pomXK13A | Strain with a replacement of pomX with the pomXK13A allele |
| Strain, strain background (M. xanthus) | SA9732 | this study | pomX::pomXS10A | Strain with a replacement of pomX with the pomXS10A allele |
| Strain, strain background (M. xanthus) | SA9739 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXQ7A (pDS317) | Strain expressing mCh-PomXQ7A in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9740 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXN8A (pDS318) | Strain expressing mCh-PomXN8A in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9741 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXR17A (pDS323) | Strain expressing mCh-PomXR17A in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9742 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXT22A (pDS324) | Strain expressing mCh-PomXT22A in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9743 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXS10A (pDS319) | Strain expressing mCh-PomXS10A in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9744 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXR11A (pDS320) | Strain expressing mCh-PomXR11A in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9747 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXK13A (pDS321) | Strain expressing mCh-PomXK13A in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9748 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXR15A (pDS322) | Strain expressing mCh-PomXR15A in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9749 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXK2A (pDS314) | Strain expressing mCh-PomXK2A in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9750 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXK3A (pDS315) | Strain expressing mCh-PomXK3A in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9751 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXE6A (pDS316) | Strain expressing mCh-PomXE6A in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9752 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXK13AR15A (pDS325) | Strain expressing mCh-PomXK13AR15A in a pomX deletion background |
| Strain, strain background (M. xanthus) | SA9753 | this study | ΔmglA; ΔpomX; attB::Pmxan0635 mCh-pomXK13AR15A (pDS325) | Strain expressing mCh-PomXK13AR15A in a non-motile pomX deletion background |
| Strain, strain background (M. xanthus) | SA9754 | this study | ΔmglA; ΔpomZ; ΔpomX; Pmxan0635 mCh-pomX (pAH53); mxan18-19::Pmxan0635 pomZD90A (pDS80) | Strain expressing mCh-PomX in a non-motile pomX and pomZ deletion background that expresses PomZD90A. |
| Strain, strain background (M. xanthus) | SA9755 | this study | Wild-type; attB::Pmxan0635 mCh-pomXC (pDS329) | Strain expressing mCh-PomXC in a WT background |
| Strain, strain background (M. xanthus) | SA9756 | this study | ΔpomY; attB::Pmxan0635 mCh-pomXC (pDS329) | Strain expressing mCh-PomXC in a pomY deletion background |
| Strain, strain background (M. xanthus) | SA9757 | this study | ΔpomZ; attB::Pmxan0635 mCh-pomXC (pDS329) | Strain expressing mCh-PomXC in a pomZ deletion background |
| Strain, strain background (M. xanthus) | SA9762 | this study | ΔpomX; attB::Pmxan0635 mCh-pomXC (pDS329) | Strain expressing mCh-PomXC in a pomX deletion background |
| Recombinant DNA reagent | pAH27 (plasmid) |
https://doi.org/10.1016/j.devcel.2017.04.011 | Construct for in-frame deletion of pomX, KmR | |
| Recombinant DNA reagent | pAH53 (plasmid) |
https://doi.org/10.1016/j.devcel.2017.04.011 | Pmxan0635 mCh-pomX, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS1 (plasmid) |
https://doi.org/10.1016/j.devcel.2017.04.011 | Construct for in-frame deletion of pomY, KmR | |
| Recombinant DNA reagent | pDS7 (plasmid) |
https://doi.org/10.1016/j.devcel.2017.04.011 | PpilA pomY-mCh, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS16 (plasmid) |
https://doi.org/10.1016/j.devcel.2017.04.011 | Construct for in-frame deletion of pomY and pomZ, KmR | |
| Recombinant DNA reagent | pDS80 (plasmid) |
https://doi.org/10.1016/j.devcel.2017.04.011 | Pmxan0635 pomZD90A, mxan_18–19 intergenic region, TcR | |
| Recombinant DNA reagent | pEMR3 (plasmid) |
https://doi.org/10.1016/j.devcel.2017.04.011 | Overexpression of PomX-His6, KmR | |
| Recombinant DNA reagent | pKA1 (plasmid) |
DOI: 10.1111/mmi.12094 | Construct for in-frame deletion of pomZ, KmR | |
| Recombinant DNA reagent | pKA3 (plasmid) |
DOI: 10.1111/mmi.12094 | Overexpression of His6-PomZ, KmR | |
| Recombinant DNA reagent | pKA43 (plasmid) |
DOI: 10.1111/mmi.12094 | Pmxan0635 pomZD90A-mCh, Mx8 attB, TcR | |
| Recombinant DNA reagent | pMAT12 (plasmid) |
https://doi.org/10.1016/j.devcel.2017.04.011 | Construct for in-frame deletion of pomZ and pomX, KmR | |
| Recombinant DNA reagent | pAH152 (plasmid) |
this study | Overexpression of PomXC-His6, KmR | |
| Recombinant DNA reagent | pSL16 (plasmid) |
DOI: 10.1038/emboj.2011.291 | Construct for in-frame deletion of mglA, KmR | |
| Recombinant DNA reagent | pUT18 (plasmid) |
https://doi.org/10.1073/pnas.95.10.5752 | BACTH plasmid | |
| Recombinant DNA reagent | pUT18C (plasmid) |
https://doi.org/10.1073/pnas.95.10.5752 | BACTH plasmid | |
| Recombinant DNA reagent | pKT25 (plasmid) |
https://doi.org/10.1073/pnas.95.10.5752 | BACTH plasmid | |
| Recombinant DNA reagent | pKNT25 (plasmid) |
https://doi.org/10.1073/pnas.95.10.5752 | BACTH plasmid | |
| Recombinant DNA reagent | pAH154 (plasmid) |
this study | Pmxan0635 mCh-pomXN, Mx8 attB, KmR | |
| Recombinant DNA reagent | pAH157 (plasmid) |
this study | Overexpression of PomXN-His6, KmR | |
| Recombinant DNA reagent | pAH165 (plasmid) |
this study | Overexpression of PomXN_K13AR15A-His6, KmR | |
| Recombinant DNA reagent | pDS100 (plasmid) |
this study | BACTH plasmid for pomZ (pUT18C), AmpR | |
| Recombinant DNA reagent | pDS103 (plasmid) |
this study | BACTH plasmid for pomX (pUT18C), AmpR | |
| Recombinant DNA reagent | pDS106 (plasmid) |
this study | BACTH plasmid for pomX (pKT25), KmR | |
| Recombinant DNA reagent | pDS109 (plasmid) |
this study | BACTH plasmid for pomZ (pUT18), AmpR | |
| Recombinant DNA reagent | pDS110 (plasmid) |
this study | BACTH plasmid for pomX (pUT18), AmpR | |
| Recombinant DNA reagent | pDS114 (plasmid) |
this study | BACTH plasmid for pomX (pKNT25) KmR | |
| Recombinant DNA reagent | pDS115 (plasmid) |
this study | BACTH plasmid for pomZD90A (pUT18C), AmpR | |
| Recombinant DNA reagent | pDS117 (plasmid) |
this study | BACTH plasmid for pomZD90A (pUT18), AmpR | |
| Recombinant DNA reagent | pDS120 (plasmid) |
this study | BACTH plasmid for pomY (pUT18C), AmpR | |
| Recombinant DNA reagent | pDS122 (plasmid) |
this study | BACTH plasmid for pomY (pUT18), AmpR | |
| Recombinant DNA reagent | pDS184 (plasmid) |
this study | BACTH plasmid for pomXΔ2-21 (pUT18), AmpR | |
| Recombinant DNA reagent | pDS185 (plasmid) |
this study | BACTH plasmid for pomXΔ2-21 (pUT18C), AmpR | |
| Recombinant DNA reagent | pDS186 (plasmid) |
this study | BACTH plasmid for pomXΔ2-21 (pKT25), KmR | |
| Recombinant DNA reagent | pDS187 (plasmid) |
this study | BACTH plasmid for pomXΔ2-21 (pKNT25) KmR | |
| Recombinant DNA reagent | pDS188 (plasmid) |
this study | BACTH plasmid for pomXC (pUT18), AmpR | |
| Recombinant DNA reagent | pDS189 (plasmid) |
this study | BACTH plasmid for pomXC (pUT18C), AmpR | |
| Recombinant DNA reagent | pDS190 (plasmid) |
this study | BACTH plasmid for pomXC (pKT25), KmR | |
| Recombinant DNA reagent | pDS191 (plasmid) |
this study | BACTH plasmid for pomXC (pKNT25) KmR | |
| Recombinant DNA reagent | pDS192 (plasmid) |
this study | BACTH plasmid for pomXN (pUT18), AmpR | |
| Recombinant DNA reagent | pDS193 (plasmid) |
this study | BACTH plasmid for pomXN (pUT18C), AmpR | |
| Recombinant DNA reagent | pDS194 (plasmid) |
this study | BACTH plasmid for pomXN (pKT25), KmR | |
| Recombinant DNA reagent | pDS195 (plasmid) |
this study | BACTH plasmid for pomXN (pKNT25) KmR | |
| Recombinant DNA reagent | pDS232 (plasmid) |
this study | Overexpression of PomXN-Strep, KmR | |
| Recombinant DNA reagent | pDS252 (plasmid) |
this study | Pmxan0635 mCh-pomXN, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS253 (plasmid) |
this study | BACTH plasmid for pomXN_K13AR15A (pUT18), AmpR | |
| Recombinant DNA reagent | pDS254 (plasmid) |
this study | BACTH plasmid for pomXN_K13AR15A (pUT18C), AmpR | |
| Recombinant DNA reagent | pDS255 (plasmid) |
this study | BACTH plasmid for pomXN_K13AR15A (pKT25), KmR | |
| Recombinant DNA reagent | pDS256 (plasmid) |
this study | BACTH plasmid for pomXN_K13AR15A (pKNT25) KmR | |
| Recombinant DNA reagent | pDS257 (plasmid) |
this study | BACTH plasmid for pomXN_Δ2-21 (pUT18), AmpR | |
| Recombinant DNA reagent | pDS258 (plasmid) |
this study | BACTH plasmid for pomXN_Δ2-21 (pUT18C), AmpR | |
| Recombinant DNA reagent | pDS259 (plasmid) |
this study | BACTH plasmid for pomXN_Δ2-21 (pKT25), KmR | |
| Recombinant DNA reagent | pDS260 (plasmid) |
this study | BACTH plasmid for pomXN_Δ2-21 (pKNT25) KmR | |
| Recombinant DNA reagent | pDS303 (plasmid) |
this study | nat. site codon exchange for pomXK2A, KmR | |
| Recombinant DNA reagent | pDS304 (plasmid) |
this study | nat. site codon exchange for pomXK3A, KmR | |
| Recombinant DNA reagent | pDS305 (plasmid) |
this study | nat. site codon exchange for pomXE6A, KmR | |
| Recombinant DNA reagent | pDS306 (plasmid) |
this study | nat. site codon exchange for pomXQ7A, KmR | |
| Recombinant DNA reagent | pDS307 (plasmid) |
this study | nat. site codon exchange for pomXN8A, KmR | |
| Recombinant DNA reagent | pDS308 (plasmid) |
this study | nat. site codon exchange for pomXS10A, KmR | |
| Recombinant DNA reagent | pDS309 (plasmid) |
this study | nat. site codon exchange for pomXR11A, KmR | |
| Recombinant DNA reagent | pDS310 (plasmid) |
this study | nat. site codon exchange for pomXK13A, KmR | |
| Recombinant DNA reagent | pDS311 (plasmid) |
this study | nat. site codon exchange for pomXR15A, KmR | |
| Recombinant DNA reagent | pDS312 (plasmid) |
this study | nat. site codon exchange for pomXR17A, KmR | |
| Recombinant DNA reagent | pDS313 (plasmid) |
this study | nat. site codon exchange for pomXT22A, KmR | |
| Recombinant DNA reagent | pDS314 (plasmid) |
this study | Pmxan0635 mCh-pomXK2A, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS315 (plasmid) |
this study | Pmxan0635 mCh-pomXK3A, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS316 (plasmid) |
this study | Pmxan0635 mCh-pomXE6A, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS317 (plasmid) |
this study | Pmxan0635 mCh-pomXQ7A, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS318 (plasmid) |
this study | Pmxan0635 mCh-pomXN8A, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS319 (plasmid) |
this study | Pmxan0635 mCh-pomXS10A, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS320 (plasmid) |
this study | Pmxan0635 mCh-pomXR11A, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS321 (plasmid) |
this study | Pmxan0635 mCh-pomXK13A, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS322 (plasmid) |
this study | Pmxan0635 mCh-pomXR15A, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS323 (plasmid) |
this study | Pmxan0635 mCh-pomXR17A, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS324 (plasmid) |
this study | Pmxan0635 mCh-pomXT22A, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS325 (plasmid) |
this study | Pmxan0635 mCh-pomXK13AR15A, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS329 (plasmid) |
this study | Pmxan0635 mCh-pomXC, Mx8 attB, KmR | |
| Recombinant DNA reagent | pDS333 (plasmid) |
this study | Overexpression of PomXC-Strep, KmR | |
| Recombinant DNA reagent | pEMR1 (plasmid) |
this study | Overexpression of PomY-His6, KmR | |
| Recombinant DNA reagent | pSH1 (plasmid) |
this study | nat. site codon exchange for pomXK13AR15A, KmR | |
| Recombinant DNA reagent | pSH36 (plasmid) |
this study | BACTH plasmid for pomXK13AR15A (pKNT25) KmR | |
| Recombinant DNA reagent | pSH37 (plasmid) |
this study | BACTH plasmid for pomXK13AR15A (pKT25), KmR | |
| Recombinant DNA reagent | pSH38 (plasmid) |
this study | BACTH plasmid for pomXK13AR15A (pUT18), AmpR | |
| Recombinant DNA reagent | pSH39 (plasmid) |
this study | BACTH plasmid for pomXK13AR15A (pUT18C), AmpR | |
| Recombinant DNA reagent | pSH58 (plasmid) |
this study | Overexpression of PomXK13AR15A-His6, KmR | |
| Sequence-based reagent | pomX BTH fwd XbaI | this study | PCR primer | 5’-GCGTCTAGAGATGAAGAAAGCCTTTGAAC-3’ |
| Sequence-based reagent | pomX BTH rev KpnI | this study | PCR primer | 5’-GCGGGTACCCGGCGCACCGTGGCCTGAC-3’ |
| Sequence-based reagent | pomY BTH fwd XbaI | this study | PCR primer | 5’-GCGTCTAGAGGTGAGCGACGAGCGTCCG-3’ |
| Sequence-based reagent | pomY BTH rev KpnI | this study | PCR primer | 5’-GCGGGTACCCGAGCGGCGAAGTATTTGTG-3’ |
| Sequence-based reagent | pomZ BTH fwd XbaI | this study | PCR primer | 5’-GCGTCTAGAGATGGAAGCGCCGACGTAC-3’ |
| Sequence-based reagent | pomZ BTH rev KpnI | this study | PCR primer | 5’-GCGGGTACCCGGCCGGCCTGCTGGGTGCC-3’ |
| Sequence-based reagent | pomXΔ2–21 BTH fwd XbaI | this study | PCR primer | 5’-GCGTCTAGAGATGACGGGCCTCGTCGACCCC-3’ |
| Sequence-based reagent | pomXC BTH fwd XbaI | this study | PCR primer | 5’-GCGTCTAGAGATGGCCACCGTGGCGGAGGCG-3’ |
| Sequence-based reagent | pomXN BTH rev KpnI | this study | PCR primer | 5’-GCGGGTACCCGGGGCAGCGGCTCCGGGCG-3’ |
| Sequence-based reagent | 0636 up fwd | this study | PCR primer | 5’-GCGGGATCCGTCACCCCAAGCCATTC-3’ |
| Sequence-based reagent | PomX K2A rev native | this study | PCR primer | 5’-CAAAGGCTTTCGCCATGGTTCTCAG-3’ |
| Sequence-based reagent | PomX K2A fwd native | this study | PCR primer | 5’-CTGAGAACCATGGCGAAAGCCTTTG-3’ |
| Sequence-based reagent | 0636 HindIII rev stop | this study | PCR primer | 5’-GCGAAGCTTTCAGCGCACCGTGGCCTGAC-3’ |
| Sequence-based reagent | PomX K3A rev native | this study | PCR primer | 5’-CTGTTCAAAGGCCGCCTTCATGGTTC-3’ |
| Sequence-based reagent | PomX K3A fwd native | this study | PCR primer | 5’-GAACCATGAAGGCGGCCTTTGAACAG-3’ |
| Sequence-based reagent | PomX E6A rev | this study | PCR primer | 5’-GGACACGTTCTGCGCAAAGGCTTTCTT-3’ |
| Sequence-based reagent | PomX E6A fwd | this study | PCR primer | 5’-AAGAAAGCCTTTGCGCAGAACGTGTCC-3’ |
| Sequence-based reagent | PomX Q7A rev | this study | PCR primer | 5’-GCGGGACACGTTCGCTTCAAAGGCTTT-3’ |
| Sequence-based reagent | PomX Q7A fwd | this study | PCR primer | 5’-AAAGCCTTTGAAGCGAACGTGTCCCGC-3’ |
| Sequence-based reagent | PomX N8A rev | this study | PCR primer | 5’-GGCGCGGGACACCGCCTGTTCAAAGGC-3’ |
| Sequence-based reagent | PomX N8A fwd | this study | PCR primer | 5’-GCCTTTGAACAGGCGGTGTCCCGCGCC-3’ |
| Sequence-based reagent | PomX S10A rev | this study | PCR primer | 5’-CGGCTTGGCGCGCGCCACGTTCTGTTC-3’ |
| Sequence-based reagent | PomX S10A fwd | this study | PCR primer | 5’-GAACAGAACGTGGCGCGCGCCAAGCCG-3’ |
| Sequence-based reagent | PomX R11A rev | this study | PCR primer | 5’-GCGCGGCTTGGCCGCGGACACGTTCTG-3’ |
| Sequence-based reagent | PomX R11A fwd | this study | PCR primer | 5’-CAGAACGTGTCCGCGGCCAAGCCGCGC-3’ |
| Sequence-based reagent | PomX K13A rev | this study | PCR primer | 5’-GCGGAGGCGCGGCGCGGCGCGGGACAC-3’ |
| Sequence-based reagent | PomX K13A fwd | this study | PCR primer | 5’-GTGTCCCGCGCCGCGCCGCGCCTCCGC-3’ |
| Sequence-based reagent | PomX R15A rev | this study | PCR primer | 5’-GCCCAGGCGGAGCGCCGGCTTGGCGCG-3’ |
| Sequence-based reagent | PomX R15A fwd | this study | PCR primer | 5’-CGCGCCAAGCCGGCGCTCCGCCTGGGC-3’ |
| Sequence-based reagent | PomX R17A rev | this study | PCR primer | 5’-CAGCGCGCCCAGCGCGAGGCGCGGCTT-3’ |
| Sequence-based reagent | PomX R17A fwd | this study | PCR primer | 5’-AAGCCGCGCCTCGCGCTGGGCGCGCTG-3’ |
| Sequence-based reagent | PomX T22A rev | this study | PCR primer | 5’-GTCGACGAGGCCCGCCAGCGCGCCCAG-3’ |
| Sequence-based reagent | PomX T22A fwd | this study | PCR primer | 5’-CTGGGCGCGCTGGCGGGCCTCGTCGAC-3’ |
| Sequence-based reagent | PomX K13AR15A rev | this study | PCR primer | 5’-CAGAACGTGTCCCGCGCCGCGCCGGCCCTCCGCCTGGGCGCGCTG-3’ |
| Sequence-based reagent | PomX K13AR15A fwd | this study | PCR primer | 5’-CAGCGCGCCCAGGCGGAGGGCCGGCGCGGCGCGGGACACCTTCTG-3’ |
| Sequence-based reagent | mCherry XbaI fwd | this study | PCR primer | 5’-GCGTCTAGAGTGAGCAAGGGCGAGGAG-3’ |
| Sequence-based reagent | PomX K2A rev | this study | PCR primer | 5’-TTCAAAGGCTTTCGCCATGGCTCCGCC-3’ |
| Sequence-based reagent | PomX K2A fwd | this study | PCR primer | 5’-GGCGGAGCCATGGCGAAAGCCTTTGAA-3’ |
| Sequence-based reagent | KA348 | this study | PCR primer | 5’-GCCAAGCTTTCAGCGCACCGTGGCCTG-3’ |
| Sequence-based reagent | PomX K3A fwd | this study | PCR primer | 5’-GGAGCCATGAAGGCGGCCTTTGAACAG-3’ |
| Sequence-based reagent | PomX K3A rev | this study | PCR primer | 5’-CTGTTCAAAGGCCGCCTTCATGGCTCC-3’ |
| Sequence-based reagent | AH142 | this study | PCR primer | 5’-GGAATTCCATATGGCCACCGTGGCGGAGGCG-3’ |
| Sequence-based reagent | KA346 | this study | PCR primer | 5’-GCCAAGCTTGCGCACCGTGGCCTGACTC-3’ |
| Sequence-based reagent | AH143 | this study | PCR primer | 5’-CCCAAGCTTGGGCAGCGGCTCCGGGCG-3’ |
| Sequence-based reagent | NdeI PomX fwd | this study | PCR primer | 5’-GGAATTCCATATGAAGAAAGCCTTTGAACAG-3’ |
| Sequence-based reagent | AH144 | this study | PCR primer | 5’-GCCAAGCTTTCAGGGCAGCGGCTCCGGGCG-3’ |
| Sequence-based reagent | KA384 | this study | PCR primer | 5’-GCGGGATCCGGCGGAGCCATGAAGAAAGCCTTTGAACAG-3’ |
| Sequence-based reagent | DS276 | this study | PCR primer | 5’-GCGAAGCTTACTTCTCGAACTGTGGGTGACTCCAGCGCACCGTGGCCTGAC-3’ |
| Sequence-based reagent | DS277 | this study | PCR primer | 5’-GCGCCATGGCCACCGTGGCGGAGGCG-3’ |
| Sequence-based reagent | PomX BspHI fwd | this study | PCR primer | 5’-GCGTCATGAAGAAAGCCTTTGAACAGAACG-3’ |
| Sequence-based reagent | PomXN rev strep-tag | this study | PCR primer | 5’-GCGAAGCTTACTTCTCGAACTGTGGGTGACTCCAGGGCAGCGGCTCCGGGCG-3’ |
| Sequence-based reagent | NdeI-PomY fwd | this study | PCR primer | 5’-GGAATTCCATATGAGCGACGAGCGTCCGGAC-3’ |
| Sequence-based reagent | PomY C-term his rev | this study | PCR primer | 5’-CGGAAGCTTAGCGGCGAAGTATTTGTGC-3’ |
| Sequence-based reagent | AH141 | this study | PCR primer | 5’-GCGGGATCCGGCGGAGCCGCCACCGTGGCGGAGGCG-3’ |
| Antibody | α-PomX (rabbit, polyclonal) | https://doi.org/10.1016/j.devcel.2017.04.011 | Western Blot (1:15000) | |
| Antibody | α-PomY (rabbit, polyclonal) | https://doi.org/10.1016/j.devcel.2017.04.011 | Western Blot (1:15000) | |
| Antibody | α-PomZ (rabbit, polyclonal) | DOI: 10.1111/mmi.12094 | Western Blot (1:10000) | |
| Antibody | α-PilC (rabbit, polyclonal) | DOI:10.1111/j.1365–2958.2009.06891.x | Western Blot (1:3000) | |
| Antibody | α-mCherry (rabbit, polyclonal) | Biovision | Cat# 5993 | Western Blot (1:10000) |
| Antibody | horseradish-conjugated α-rabbit immunoglobulin G (goat,polyclonal) |
Sigma-Aldrich | Cat# A0545-1ML | Western Blot (1:25000) |
| Peptide, recombinant protein | PomXNPEP | Thermo Scientific | MKKAFEQNVSRAKPRLRLGALT | |
| Peptide, recombinant protein | PomXNPEPK13AR15A | Thermo Scientific | MKKAFEQNVSRAAPALRLGALT | |
| Software, algorithm | Metamorph_v 7.5 | Molecular Devices | ||
| Software, algorithm | Oufti | DOI: 10.1111/mmi.13264 | http://www.oufti.org/ | |
| Software, algorithm | Matlab R2018a | MathWorks | ||
| Commercial assay or kit | Luminata Forte | Fisher scientific | Cat# 10394675 |