Figure 3. IL-4/13 levels do not change during resolution of atherosclerosis.

(A) Quantification of CCL2, IL-4, and IL-13 protein levels in aortic arch plaques of Ldlr^-/- BL mice or further treated for lipid lowering for 3 weeks (Res). (B) Experimental design. (C) CD68-positive area in aortic roots. (D) Gating scheme of aortic arches single-cell suspensions and (E) quantification of IL-4 (eGFP+) producing cells in aortic arches. **p<0.01 and *p<0.05 determined by an unpaired t-test (two-tailed).

Figure 3—figure supplement 1. Il13 expression in plaques during disease progression and resolution on a single cell level.

Il13 gene expression in plaque immune cells (from Sharma et al., 2020).