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. 2021 Mar 1;11(3):997–1008.

Figure 5.

Figure 5

Silencing ASIC1 inhibits the migration of U87MG cells. (A) Representative Western blot bands and (B) quantification of the ASIC1 expression showing the transfection efficiency of siRNA-ASIC1 in U87MG cells. (C) Representative micrographs and (D) statistical results showing that knockdown of ASIC1 has no obvious effect on the proliferation of U87MG cells both at 24 h and 72 h without or with the treatment of extracellular weak acid. (E) Representative micrographs and (F) statistical results showing the effect of siRNA-Control or siRNA-ASIC1 on acid-induced migration of U87MG cells by wound-healing assay. (G) Representative micrographs and (H) statistical results showing the effects of siRNA-Control or siRNA-ASIC1 on acid-induced migration of U87MG cells by transwell assay. Data are expressed as mean ± SEM. n=3-5. Black bar =250 µm. White bar =100 µm. *P < 0.05, **P < 0.01, ***P < 0.001, by one-way ANOVA followed by Tukey’s multiple comparison test.