Table 1.

Reversal potential and conductance of VDAC1 open and αSyn-blocked states measured in different CaCl₂ and KCl gradients. Reversal potential values are corrected for the liquid-junction potential. The ratios of anion to cation permeabilities, P₋/P₊, and currents, I₋/I₊ are calculated according to Eqs. (1–2) and (6), respectively, at 0 mV. Each data point is a mean ± S.E.. Number of independent experiments is shown in brackets. All conditions are as in Fig. 3.

Salt gradient cis/trans, mM	Single channel conductance, nS	Reversal potential, mV	Permeability ratio, P−/P+	Ion currents ratio at 0 mV, I−/I+
CaCl2 gradient
500/100 Open (10)	1.40 ± 0.2	25.5 ± 3.4	11.5 ± 3.7	7.5 ± 2.7
500/100 Blocked (3)	0.58 ± 0.10	24.8 ± 4.1	11.0 ± 4.2	7.0 ± 2.4
100/500 Open (11)	1.58 ± 0.33	−26.4 ± 2.2	11.9 ± 2.6	8.3 ± 2.1
100/500 Blocked (3)	0.55 ± 0.03	−27.0 ± 1.1	12.5 ± 1.8	8.9 ± 1.1
150/30 Open (11)	0.58 ± 0.12	31.4 ± 0.9	40.7 ± 9.8	26.9 ± 7.3
150/30 Blocked (4)	0.36 ± 0.09	−5.5 ± 3.2	0.60 ± 0.20	0.59 ± 0.20
30/150 Open (10)	0.56 ± 0.16	−28.4 ± 1.0	20.3 ± 4.3	13.9 ± 2.4
30/150 Blocked (4)	0.39 ± 0.06	8.9 ± 1.4	0.35 ± 0.07	0.39 ± 0.08
KCl gradient
300/60 Open (12)	0.59 ± 0.09	18.5 ± 3.2	3.7 ± 1.1	2.97 ± 0.18
300/60 Blocked (3)	0.39 ± 0.06	−17.7 ± 2.5	0.31 ± 0.06	0.35 ± 0.06