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. 2021 Mar 25;11:6842. doi: 10.1038/s41598-021-83240-x

Figure 1.

Figure 1

c407 activity in HeLa cells and Human nasal primary cells. (A) Whole cell patch-clamp experiments. Mean (SEM) CFTR-related current/voltage relationships obtained in HeLa cells stably expressing F508del-CFTR after 48 h incubation with NaCl 0.9% (empty circle, n = 5); c407 1 µM (grey circle, n = 14) and 10 µM (black circle, n = 9). Current densities normalized to cell capacitance (ICFTR/C) were calculated as the differences between current values in the presence of CPT-cAMP 400 µM/IBMX 100 µM minus current values after inhibition with CFTRinh-172 5 µM. (B) Summary of the mean CFTR current amplitudes recorded at − 60 mV and normalized to cell capacitance of the experiments in A. Results are also shown for Wild Type (WT) CFTR cells (black dotted; n = 10). Wilcoxon-test statistics are shown. (C) Immunoblot of HeLa cells stably expressing F508del-CFTR after incubation with c407 at 10 µM for 48 h, and NaCl 0.9% as negative control. Arrows indicate CFTR immature core glycosylated band B and complex glycosylated mature band C. CFTR was immunoblotted with monoclonal CFTR antibody 660, at 1:1000. Representative experiments of n = 12. (D) Summary of CFTR expression quantification. CFTR expression is evaluated by the ratio C/B + C. The ratio is significantly higher in F508del-HeLa cells incubated with c407 at 10 µM (n = 12 different experiments) versus F508del-HeLa cells incubated with control water (n = 15); p = 0.015 (Wilcoxon non parametric test).