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. 2021 Mar 25;11:6845. doi: 10.1038/s41598-021-85592-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended-culture approach results. (a) Description of strict anaerobic genera identified in 112 sputum samples: Gram-positive genera are shown in purple, Gram-negative genera in pink, and number of species in brackets. (b) Prevalence of strict anaerobic genera identified according to patient age group (80 patients). (c) Prevalence of strict anaerobic genera identified according to patient lung function evaluated by Forced Expiratory Volume in one second (FEV₁) (80 patients).