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. 2021 Mar 25;12:1857. doi: 10.1038/s41467-021-22152-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Whisker box plots showing pilA1 gene abundance in Prochlorococcus (green) and Synechococcus (orange) in metagenomes (solid bars; n_mg = 174) and metatranscriptomes (dotted bars; n_mt = 178) generated from all filters (0.2–3 µm) obtained from 125 sampling stations of the TARA Oceans global marine survey. Abundance was calculated by dividing the sum of the abundances of pilA1 homologs assigned to each genus by the sum of total gene abundance from all reads from the sample. Data are presented by oceanic region (North Atlantic Ocean, n_mg = 23, n_mt = 21; Mediterranean Sea, n_mg = 12, n_mt = 7; Red Sea, n_mg = 6, n_mt = 3; Indic Ocean, n_mg = 27, n_mt = 22; South Atlantic Ocean, n_mg = 19, n_mt = 19; Southern Ocean, n_mg = 4, n_mt = 8; South Pacific Ocean, n_mg = 31, n_mt = 41; North Pacific Ocean, n_mg = 16, n_mt = 25; Arctic Ocean, n_mg = 36, n_mt = 32), biome (Coastal, n_mg = 35, n_mt = 33; Westerlies, n_mg = 37, n_mt = 28; Trades, n_mg = 62, n_mt = 103; Polar, n_mg = 40, n_mt = 40) and water depth (surface, n_mg = 62, n_mt = 78; deep chlorophyll maxima (DCM), n_mg = 43, n_mt = 39; mesopelagic layers, n_mg = 29, n_mt = 21). Filters from polar regions were excluded from the depth abundance analysis. Whiskers indicate variability outside the upper and lower quartiles (boxes). Exclusive median (line), average (cross) and atypical values (circles) are also indicated. Source data are provided as a Source Data file.