Fig. 3. Effects of treating the macrophages directly with 1,25D on CRIg expression.

a Macrophages matured after 3 days of monocyte culture, were treated for a further 24 h with 100 nM of 1,25D or diluent and then the CRIg mRNA levels measured by qPCR. Data are expressed as CRIg relative to GAPDH from four experiments, each conducted with cells from a different individual. b Macrophages differentiated from culturing monocyte for 5 days culture, were treated as described above. The CRIg expression was measured by western blot in three experiments, each conducted with cells from different individuals. A representative western blot is shown of CRIg and GAPDH staining of the same blot. a, b Relative expression (RE) of mRNA or protein was measured against GAPDH. P values were calculated by paired, one-tailed Student’s t-test. Significance of differences between 1,25D versus control, *P < 0.05; **P < 0.01.