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. 2021 Mar 25;12:1892. doi: 10.1038/s41467-021-22093-4

Fig. 1. Structure of the PfMyoA motor domain/PfAct1 filament assembly.

Fig. 1

a Representative micrograph (out of 6073) of JAS-stabilized PfAct1 filaments with bound PfMyoA motor domain. The scale bar is 50 nm. b Representative classes (out of 1534) from reference-free two-dimensional classification of segments showing lack of cooperative binding (full decoration). The scale bar is 20 nm. c Reconstruction of JAS-stabilized PfAct1 filaments (pink) with bound PfMyoA motor domain (gray). The pointed (-) end of the filament is up. d Local resolution mapped on the reconstruction. Values of color bar in Å. e Atomic temperature factors mapped on the refined model. Values of color bar in Å2 f–i Density maps and atomic model for all the actin binding elements of PfMyoA at the actomyosin interface. All the different actin binding elements are colored differently: The hypertrophic cardiomyopathy loop (HCM) in pink; Loop2 in yellow; Loop3 in dark gray; helix-turn-helix (HTH) in dark blue (see also Supplementary Fig. 1).