Fig. 1. Endothelial expression of Jak3 is enhanced by a hematopoietic milieu in vitro and in vivo.

a Heatmap depicting absolute transcript abundances of genes of interest obtained by RNA sequencing (RNA-seq) of primary murine endothelial cell (EC) populations extracted from the indicated vascular beds. Average FPKM values for all biological replicates (n = 2 or 3 per organ) appear in black. b Venn diagram showing number of intersecting overrepresented gene transcripts resulting from differential expression analyses of each vascular bed against all other samples (FDR < 0.05). c Bar plot of enrichment scores of the 15 most upregulated Kyoto Encyclopedia of Genes and Genomes (KEGG)^54–56 terms generated by gene set enrichment analysis (GSEA) using the log₂ fold change of the differentially expressed gene transcripts in the bone marrow vasculature compared to that of the liver, lungs, heart, and kidneys. d Heatmap depicting normalized transcript abundances of genes that contributed to the 15 most upregulated KEGG terms by GSEA. Average FPKM values for all biological replicates (n = 2 or 3 per organ) appear in black. Jak3, the most highly and differentially expressed transcript, is highlighted in red. e Janus kinase (Jak) family gene expression across the organs assayed as measured by RNA-seq. ****p value < 0.0001 and ns (not significant) > 0.05, by two-way ANOVA. f Jak family transcript expression in primary vs. cultured bone marrow ECs (BMECs) as measured by RNA-seq (n = 3 per group). Raw data were obtained from Poulos et al.²⁶ (GEO: GSE61636) and re-analyzed for this publication. ***p value < 0.001 and ns > 0.05, by unpaired, two-tailed Student’s t test. g Jak family transcript expression in BMECs cultured alone vs. in contact with hematopoietic stem and progenitor cells for 8 days as measured by RNA-seq (n = 3 per group; left). Volcano plot illustrating differential expression analysis of these data (right). Jak family gene transcripts are highlighted with enlarged dots in red; respectively, light or dark gray dots indicate gene transcripts differentially expressed above or below cutoffs, –log₁₀ (p value) < 1 and |log₂FC| < 2.5. Raw data were obtained from Poulos et al.²⁶ (GEO: GSE61636) and re-analyzed for this publication. ***p value < 0.001, **p value < 0.01, and ns (not significant) > 0.05, by unpaired, two-tailed Student’s t test. On all plots, each replicate is represented by an individual dot and error bars represent standard deviation from the mean.