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. 2021 Mar 25;11:6890. doi: 10.1038/s41598-021-86315-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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hMSCs retain their phenotype in the presence of IL-1β. (A) Quantification of the concentration of IL-1β in the cell medium upon initial addition of either control or IL-1β medium to cells and after 1 and 7 days in culture as measured by ELISA. N = 10 replicates for each condition. Parametric one-way ANOVA, Tukey multiple comparison test. (B) Quantification of DNA concentration of hMSCs after 1 and 7 days in culture. N = 27 replicates for each condition. Non-parametric Kruskal–Wallis test with Dunn’s multiple comparison test. ***p < 0.001. Box and whisker plots represent minimum to maximum. (C,D) Representative flow cytometry dot plots of forward scatter (FSC-H) versus APC fluorescence intensity for the cell surface markers CD44, CD73, CD90 and CD105 for control and IL-1β treated (10 ng/mL) hMSCs following (C) 1 day or (D) 7 days in culture. Gating strategy and analysis are in Supplementary Figure S1.