FIGURE 4.

EGF but not TGFβ depends on MAPK signaling for stimulation of migration and morphological alterations. (A) Cells were pre-treated with the indicated inhibitors or vehicle 1 h before treatment with TGFβ, EGF, or both (T + E) and images were recorded after 48 h. Growth factor-specific alterations in cell morphologies (as described in Figure 2A) are indicated by the respective colors. (B) Cells were treated as above with SB-431542 (SB), erlotinib (Erlo), LY-294002 (LY), MK-2206 (MK), U0126 (U0), BI-853520 (BI), Y-27632 (Y), or vehicle (V) plus the respective growth factors, and distance to nearest neighbors was assessed using Image J. Data are shown as scatter plots and mean (yellow line) of >100 individual cells. The mean diameter of a non-treated cell (37 μm) is shown as a red horizontal line and was chosen as cut-off for the mean to describe cell scattering (Bakiri et al., 2015; Selvaraj et al., 2015; Hyakusoku et al., 2016).