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Effect of E6/E7/LMP1 on colony formation, in soft agar, in (A) MCF7 and (B) MDA-MB-231 cell lines. As shown, E6/E7/LMP1 induces colony formation of MCF7 and MDA-MB-231 cells in comparison with their matched control cell lines; in contrast, E6/E7 and LMP1 are unable to incite HNME cells to form a colony in soft agar (data not shown). Colonies were counted manually and expressed as mean ± SEM.
