Fig. 7. Summary of the XL-MS and HDX-MS experiments.

Relative fractional uptake (RFU) differences export on the crystal structure of the complex between Rtt106p-M and Bcd1p_FL determined by hydrogen deuterium exchange mass spectrometry (HDX-MS)_. Export is realized for 2 min deuteration experiments. Differences are color scaled on Bcd1p_FL from blue (deprotection) to red (protection) upon Rtt106p-M binding (−10% to 10% RFU difference range). Differences are color scaled on Rtt106p-M from green (deprotection) to magenta (protection) upon Bcd1p_FL binding (−10% to 10% RFU difference range). The Cα-Cα distances of intercross-linked peptides are represented with black dotted lines. Orange residues and secondary structures represent the most affected regions on Bcd1p_FL while the purple ones represent the most affected regions on Rtt106p-M.