Table 3.

Cross-linked sites observed with different protein:BS3 (d0/d4) ratios for the complex Bcd1p_FL:Rtt106p-M.

Cross-linked proteins	Linkage sites	Ratio 1:50	Ratio 1:100	Distancea Cα–Cα (Å)
Bcd1pFL–Bcd1pFL	K16–K62		√	N.C.
	K16–K88	√	√	N.C.
	K16–S106	√		N.C.
	K16–K109			N.C.
	K16–K151		√	N.C.
	K16–K258	√	√	N.C.
	K62–K80		√	N.C.
	K80–K151	√		N.C.
	K87–K151	√		N.C.
	K140–K151	√		15.4
	K188–K151	√	√	10.1
Rtt106p-M–Bcd1pFL	S177–K151	√	√	19.4

Identified cross-linked sites are represented for all tested ratios (technical duplicates).

^aCα–Cα distances are indicated according to the crystal structure (N.C. for “not crystallized” mention is indicated for cross-links involving at least one site that has not been crystallized).