|
Bacterial Strains
|
|
S. aureus USA300 |
(CDC, 2003) |
Community-acquired methicillin resistant S. aureus, parental strain |
|
S. aureus USA300-Lux
|
(Plaut et al., 2013) |
pRP1190::luxCDABE (gapA-Pro, Cmr) |
|
P. aeruginosa LESB58 |
(Cheng et al., 1996) |
Liverpool epidemic strain (LES)B58, isolated from a cystic fibrosis patient |
|
P. aeruginosa LESB58-Lux
|
(Pletzer et al., 2018) |
pUCP::luxCDABE (rpoZ-Pro, Gmr) |
|
P. aeruginosa LESB65 |
(Carter et al., 2010) |
LESB65, isolated from a cystic fibrosis patient |
|
P. aeruginosa LESB65-Lux
|
This study |
Chromosomal::luxCDABE (P1-Pro, Gmr) |
|
Recombinant DNA/plasmids
|
| pUC-mini-Gm-lux-P1-Pro |
(Damron et al., 2013) |
pUC18T::mini-Tn7T::luxCDABE::P1-Pro, Gmr
|
| pTNS2 |
(Choi et al., 2005) |
pTNS::lacI-Pro::RSF, Kanr
|
|
Chemicals and Peptides
|
| IDR-1018 |
(Achtman et al., 2012) |
VRLIVAVRIWRR-NH2
|
| IDR-1002 |
(Nijnik et al., 2010) |
VQRWLIVWRIRK-NH2
|
| DJK-5 |
(de la Fuente-Nunez et al., 2015) |
vqwrairvrvir-NH2
|
| L-012 sodium salt |
Millipore Sigma |
Cat#SML2236 |
| Sodium alginate |
Sigma |
Cat#W201502 |
| TMB solution |
eBioscience |
Cat#00-4201-56 |
| Avidin horseradish peroxidase |
eBioscience |
Cat#18-4100 |
| Cytotoxicity detection kit |
Roche |
Cat#11644793001 |
|
Antibodies pairs for ELISA
|
| Murine IL-6 |
eBioscience |
Cat #14-7061, 13-7062 |
| Murine KC (CXCL1) |
R&D |
Cat#MAB453, BAF275 |
| Human IL-6 |
eBioscience |
Cat#14-7069, 13-7068 |
| Human IL-8 |
Invitrogen |
Cat#AHC0982, AHC0789 |
|
Tissue Culture Reagents
|
| MEM |
Gibco |
Cat#11090081 |
| L-glutamine (200mM) |
Gibco |
Cat#25030081 |
| Trypsin-EDTA (0.25%) |
Gibco |
Cat#25200056 |
| PBS (pH7.4) |
Gibco |
Cat#10010023 |
|
Experimental Models: Mouse Strains
|
| CD-1 mice |
Charles Rivers Laboratories, Inc |
Crl : CD1(IGS) |
| C57Bl/6 mice |
Charles Rivers Laboratories, Inc |
C57Bl/6:6NCrl |
|
Software and Algorithms
|
| GraphPad Prism 8.0 |
La Jolla, CA |
v8.0 |
| KC4 |
Bio-Tek Instruments, VT |
v3.0 |
| Living Image Lumina Series |
PerkinElmer, MA |
v3.1 |