Table 6.
Conjugation frequency and AMR profile of transconjugants compared to donors (tested isolates) using E. coli J53 as a recipient.
| Donor Strain | IsolateNumber | Conjugation frequency | Transferable AMR | Non-transferable AMR |
|---|---|---|---|---|
| E. coli | 11.1 12.1 57.1 58.1 61.1 b 82.1 89.1 113.1 c |
4 X 10-5
13 X 10-3 14 X 10-5 3 X 10-1 3 X 10-4 5 X 10-2 Non-transferable 3 X 10-4 |
AML, FOX, STR, TIO, CHL
a
, CRO, TET AML, FOX, TIO, CRO AML, STR, TET AML, FOX, GEN, STR, TIO, CHL, CRO, TET AML, FOX AML, FOX, GEN, STR, TIO, CHL a , CRO N/A AML, FOX, CRO, TIO |
NAL NAL, CHL, TET FOX, TIO, CRO - TIO, CRO, GEN, STR, TET, TIO TET AML, FOX, STR, TIO, CHL, CRO, TET NAL, STR |
|
Salmonella
(Heidelberg) |
119.2 | 9 X 10-2 | AML, TIO a , CRO a , TET a | STR |
Antimicrobial abbreviation: AML, Amoxicillin; FOX, Cefoxitin; CRO, Ceftriaxone; GEN, Gentamicin; NAL, Nalidixic acid; CHL, Chloramphenicol; TET, Tetracycline; TIO, Ceftiofur; STR, Streptomycin.
a
Results were interpreted as intermediate (between resistant and sensitive) in recipient E. coli strains while donor strains (original isolates) were resistant to these antimicrobial agents.
b
Donor strains produced colicin which is lethal to recipient strains. Trypsin was added into the media to rescue recipient strains.
c
Helper strains were required to help transfer plasmid from donor strains to recipient strains.