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. 2021 Mar 12;9:648461. doi: 10.3389/fcell.2021.648461

Figure 3.

Figure 3

Treatment with FK506 blocks the induction of lipid peroxidation product 4-HNE by noise. (A) Surface preparations show immunolabeling for 4-HNE (red) in outer hair cells (OHCs) assessed 3 h after the completion of exposure. Noise-increased 4-HNE is inhibited by treatment with FK506. Green, phalloidin labeling of sensory hair cells. Images were taken from the basal turn corresponding to sensitivity to 22–32 kHz, and each figure is representative of five individual mice for each condition. Scale bar = 10 μm. (B) Quantification of relative 4-HNE immunolabeling intensity in grayscale in OHCs normalized to dimethyl sulfoxide control mice confirms inhibition by treatment with FK506. Data are presented as individual points with means ± SD; each condition was examined in one ear per mouse; ***p < 0.001. (C) This representative image shows very strong 4-HNE immunolabeling in structurally damaged OHCs assessed 3 h after exposure. The image was taken from the lower basal turn corresponding to sensitivity to 45 kHz. (C′) For better visualization, three OHCs were enlarged and presented in the right panels with intact OHCs (1), structurally damaged OHCs (2), and scars of lost OHCs (3). This figure is representative of five individual animals per group. Scale bar = 10 μm.