Figure 4.

Treatment with FK506 increases LC3B in outer hair cells (OHCs). (A) Surface preparations show that immunolabeling for LC3B (red) in OHCs was increased after treatment with FK506 when assessed 3 h after the completion of noise exposure. Green, phalloidin labeling of sensory hair cells. Images were taken from the basal turn corresponding to sensitivity to 22–32 kHz, and each figure is representative of three to five individual mice for each condition. Scale bar = 10 μm. (B) Quantification of relative LC3B immunolabeling intensity in grayscale in OHCs normalized to dimethyl sulfoxide control mice confirms enhancement by treatment with FK506. Data are presented as individual points with means ± SD; each condition was examined in one ear per mouse; **p < 0.01.