TABLE 1.

Recent scRNA-seq studies profiling the non-hematopoietic compartment of the murine bone marrow.

Publication	Isolation approach	Source	Digestion	Enzymes	Library platform	Analyzed cells
Baccin et al., 2020	- Negative selection approach. - Bone marrow and bone fractions.	Femurs, tibiae, hips and spines	Undigested and enzymatically digested	Collagenase II and Dispase	10× Genomics, FACS-indexed, LCM-seq	7,497 (2,540 non-hematopoietic)
Baryawno et al., 2019	- Negative selection approach. - Ter119LOW,CD71LOW, CD45–, CD3–, B220–, CD19–, Gr-1–, Cd11b–. - Bone marrow and bone fractions.	Femurs and tibia	Enzymatic digestion	STEMxyme1 and Dispase II	10× Genomics	20,896
Tikhonova et al., 2019	- Positive selection approach. - VEcad-tdT, Lepr-tdT, and Col2.3-tdT animals. - Sorted tdT+CD45LOWTER119LOW. - Lepr-tdT/VEcad-tdT—Bone marrow and Col2.3-tdT—Bone marrow and bone fractions.	Femurs, tibias, and ileums	Enzymatic digestion	Liberase and DNaseI	10× Genomics	9,622
Wolock et al., 2019	- Negative selection approach. - CD45LOW, Ter119LOW, CD31– - Bone marrow and bone fractions.	Femurs, tibiae, and pelvic bones	Enzymatic digestion	Collagenase/Dispase	inDrops	2,847
Zhong et al., 2020	- Positive Selection approach. - Col2-tdT Bone marrow.	Long bones	Enzymatic digestion	Not indicated	10× Genomics	13,759