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. 2021 Mar 12;9:626316. doi: 10.3389/fcell.2021.626316

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Copyright © 2021 Coleman and Parlo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tumor vs. normal liver extra- and intra-mitochondrial citrate levels: time-course incubations fed pyruvate + malate. Mitochondria from each tissue source were incubated with 0.5 mM pyruvate/0.1 mM malate/15 mM ADP. At indicated time intervals, incubation aliquots were rapidly centrifuged through a silicone oil layer into perchloric acid. Extramitochondrial citrate was determined on the samples above, and intramitochondrial citrate was determined on samples below the silicone oil barrier. (A) Hepatoma 3924A, • extramito; ○ intramito. Normal ACI rat liver, □ extramito; ▼ intramito. (B) Hepatoma 16, • extramito; ○ intramito. Normal Buffalo rat liver, □ extramito, ▼ intramito (see: Parlo and Coleman, 1984, for details on methods).