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. 2021 Mar 12;9:626316. doi: 10.3389/fcell.2021.626316

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Copyright © 2021 Coleman and Parlo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) State 3 rates: O₂ consumption tumor vs. normal mitochondria. Duplicate incubations (30°C) contained 5 mM respiratory substrates plus 250 μM ADP addition aliquots. ADP/O ratios for all NAD⁺-linked substrates averaged 2.63; for succinate, 1.77. ADP/O ratios were unmeasurable with mitochondria from Morris hepatoma 3924A when fueled by pyruvate or citrate since ADP-initiated O₂ uptake was negligible. Data error bars are means ± SD. (B) State 3 rates: O₂ consumption pre-/post-aconitate hydratase, tumor vs. normal mitochondria, ± BTC. Incubations were performed as described for (A). 150 μM BTC additions to each incubation were made 30 s after substrate and 30 s prior to additions of 250 μM ADP. Data error bars are means ± SD.