FIGURE 8.

BTC inhibits cell growth and [³H]TdR incorporation into DNA. Exponentially growing unsynchronized or double-thymidine-block-generated G₁/S synchronized 70Z/3 murine lymphoma cultures were incubated with [³H]TdR together with the BTC concentrations indicated (see Rao and Coleman, 1989, for experimental details). Synchronized cultures were found to incorporate maximal [³H]TdR about 3 h after release from the thymidine blockade. Data show means ± SEM for three experiments (12 replicate samples/experiment). △, % of total number of unsynchronized cells; •, % of [³H]TdR incorporation into unsynchronized cells; ○, % of [³H]TdR incorporation into G₁/S synchronized cells (adapted with permission, Rao and Coleman, 1989).