Figure 2.

Differential clearance of RBCs based upon antigen copy number. (A) Twenty-four hour post-transfusion recoveries were determined (as described in Materials and Methods) for the indicated doses of antibody and for KEL_Hi, KEL_Med, or KEL_Lo RBCs. (B) The extent to which infused PUMA4 bound to the surface of transfused RBCs was determined by staining peripheral blood with anti-Ig APC, gating on the transgenic RBCs via DiO florescence, and then determining APC fluorescence on the gated population. This is a flow cytometry-based version of what is routinely referred to as the direct anti-globulin test. A representative experiment is shown, which was repeated 3 times with similar results. The experiment was repeated 3 times with similar results (representative shown); repeat experiments included in Supplemental Figures 1 and 2 .