Figure 4.

IgG2c enhances alloimmunization to KEL_Hi, KEL_Med, and KEL_Lo RBCs. Mice were treated as indicated in the experimental design (see Figure 1C ) using the indicated dose of anti-Kpb IgG2c and the indicated RBC donors. Anti-KEL IgM and IgG were tested at the indicated times by incubating serum with KEL_Hi RBCs followed by anti-IgG. Background florescence was determined by staining wild-type RBCs and subtracted to calculate adjusted MFIs, as indicated in methods. The experiment was repeated 3 times with similar results (representative shown); repeat experiments included in Supplemental Figure 4 . Data were analyzed with a repeated measures 2-way ANOVA with multiple comparisons test against PBS control. ****p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05.