Figure 5.

Enhancement of alloimmunization by IgG2c is antigen specific. Mice were treated as per the general experimental design (as in Figure 1C ) with two different RBC donors (HOD or KEL_Lo) and with two different antibodies (anti-Kp^b or anti-Fy). Serum was collected at the indicated days and tested for IgM or IgG against either HOD or KEL antigens using HOD or KEL_Hi RBCs as targets and using the appropriate isotype specific secondary antibodies (as in Materials and Methods). Each panel is labeled with regards to the RBC targets used, and therefore, the specificity of the antibody being tested. Treatments of each animal are listed below each panel. The experiment was repeated twice with similar results (representative shown); repeat experiment included in Supplemental Figure 5 .