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. 2021 Mar 12;11:630812. doi: 10.3389/fcimb.2021.630812

Figure 2.

Figure 2

PS exposure and hemolysis levels of naïve erythrocytes strongly depend on cell culture parameters. Percentage of PS-exposing cells (A) and hemolysis (normalized by hematocrit) (B) of erythrocytes cultured for 48 h in either Ringer solution at 0.4% or 4% hematocrit, or in cRPMI at 4% hematocrit. The data presented corresponds to six independent experiments performed in triplicate (N=6). Percentage of PS-exposing cells (C) and hemolysis levels (D) of erythrocytes cultured for 48 h in either Ringer solution or incomplete RPMI (iRPMI) supplemented or not with Albumax (all conditions at 4% hematocrit). The data presented corresponds to four independent experiments performed in triplicate (N=4). Individual data points represent the means of the three technical replicates for each experiment. The bars represent the mean and SD of the four to six independent experiments. Unless indicated otherwise, compare to the first condition. p > 0.05: ns; p ≤ 0.05: *; p ≤ 0.01: **; p ≤ 0.001: ***; p ≤ 0.0001: ****.