Figure 3.

Ionomycin induces PS exposure in all conditions but Albumax delays eryptosis. Erythrocytes were cultured in Ringer (+/- Albumax) or incomplete RPMI (+/- Albumax) in the presence of the calcium ionophore ionomycin (1 µM) or carrier control (DMSO). PS exposure (A) and hemolysis (B) levels were measured after 48 h. Further, PS levels were determined after 1, 4, 8, 24, and 48 h of incubation in Ringer (C) or iRPMI (D). The data presented corresponds to four independent experiments performed in triplicate (N=4). Individual data points represent the means of the three technical replicates for each experiment. The bars represent the mean and SD of the four independent experiments. p > 0.05: ns; p ≤ 0.05: *; p ≤ 0.01: **; p ≤ 0.001: ***; p ≤ 0.0001: ****. For (C, D): unless indicated otherwise, compare each timepoint to T_1h within each condition; only significant differences are indicated.