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. 2021 Mar 25;12(4):320. doi: 10.1038/s41419-021-03620-z

Fig. 1. I/R injury and hypoxia increase renal autophagy and decrease FXR expression in mice and HK2 cells.

Fig. 1

A At 48 h after I/R, the mice were euthanized, and the kidneys were collected (n = 4). Protein levels of ATG7, Becn1, LC3, and FXR were detected by immunoblotting. The relative protein levels are shown. The values for the Sham group were set to 1. B HK2 cells were exposed to hypoxia for the indicated times, and the levels of autophagy-related proteins and FXR were detected by immunoblotting. The relative levels are shown (n = 3). The level of each protein, including actin, in the 0 h hypoxia sample was set to 1. C HK2 cells were transfected with the ptf-LC3 expression plasmid, after which the cells were subjected to hypoxic conditions for 6 h. Fluorescence was imaged by confocal microscopy. The average number of LC3 II puncta per cell is shown on the right (n = 8–9). Scale bar, 20 μm. All values are presented as the mean ± SD. Statistical significance was measured using one-way ANOVA with the Bonferroni post-test. *P < 0.05, **P < 0.005.