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. 2021 Mar 25;12(4):320. doi: 10.1038/s41419-021-03620-z

Fig. 3. Autophagy is inhibited by FXR activation in hypoxia-treated HK2 cells.

Fig. 3

A, B After treatment with Baf-1 (100 nM), GW4064 (500 nM), or INT-747 (500 nM) for 1 h, HK2 cells were exposed to hypoxia for another 6 h. The protein levels of p62, LC3, and FXR were detected by immunoblotting, and the relative levels of p62/Actin, FXR/Actin, and the LC3 II/I ratio are shown in the right top and right bottom panels, respectively (n = 3). C HK2 cells were transfected with the ptf-LC3 expression plasmid. After treatment with GW4064 (500 nM) for 1 h, HK2 cells were exposed to with hypoxia for another 6 h. Fluorescence was imaged by confocal microscopy. The average number of LC3 puncta per cell is shown on the right (n = 8). All values are presented as the mean ± SD. Statistical significance was measured using one-way ANOVA with the Bonferroni post-test. *P < 0.05, **P < 0.005.