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. 2021 Mar 25;12(4):320. doi: 10.1038/s41419-021-03620-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — At 7 days after I/R, kidney samples were collected for measurements. A Protein levels of p62, LC3, Bcl2, Bax, and FXR were detected by immunoblotting. B The relative protein levels are shown, and the values for the WT-Sham group were set to 1 (n = 3–4). C Renal cortical tissues were collected for hematoxylin and eosin (H&E), periodic acid-Schiff (PAS) and Sirius Red staining to examine histology and for immunohistochemistry to examine the expression of F4/80, 3-NT, and 4-HHE. Computer-based morphometric analysis is shown (right bar graph, n = 6 in each group). All values are presented as the mean ± SD. Statistical significance was measured using one-way or two-way ANOVA with the Bonferroni post-test. *P < 0.05, **P < 0.005, ^#P < 0.05.