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. 2021 Mar 25;12:1869. doi: 10.1038/s41467-021-22076-5

Fig. 2. Cortistatin analogue 5 regulates macrophage and lymphocyte activation.

Fig. 2

Effects of Somatostatin (SST), Cortistatin (CST) and analogue 5 (A5) on the production of inflammatory cytokines and nitric oxide by LPS-activated mouse Raw 264 macrophages (a), on cell proliferation and production of Th1-type cytokines by anti-CD3-activated mouse spleen cells (b) and on the production of Th2-type cytokines by OVA-activated spleen cells isolated from mice immunised with OVA (c). Peptides were used at 100 nM and the dose-response curve for analogue 5 and cortistatin are shown in Supplementary Fig. 2a, b. Cells cultured in medium alone were used as unstimulated controls. Data are the mean ± SEM with dots representing individual values of biologically independent cell cultures, each performed in duplicate. Statistical significance between groups was assessed by paired, two-tailed Student’s t test. ***p < 0.001, ****p < 0.0001 vs. stimulated cells in the absence of peptides. Exact p-values are shown for p > 0.001. Source data are provided as a Source Data file.