Figure 3.
iCasp9/AP1903 induces apoptosis in GM-pMCs more efficiently than does HSV-TK/GCV
(A and B) Cell proliferation. Cells were cultured for 72 h in 96-well culture plates in the presence of increasing doses of GCV (A) or AP1903 (B). The culture medium served as a control. Proliferation was determined using the MTT assay. (C and D) Number of cells after suicide induction. Cells (1.0 × 105/well) were seeded in 24-well culture plates in the presence of 10 μM GCV (C) or 10 nM AP1903 (D). The culture medium served as a control. Viable cells were enumerated by direct counting of trypan-blue-stained-cells at the indicated time points. See also Figure S2. (E) GM-CSF production. Cells were cultured for 24 h in the presence of 10 μM GCV or 10 nM AP1903. GM-CSF levels in the culture supernatants were evaluated using ELISA. The medium served as a control. (A–E) Data are shown as mean ± SD of triplicate cultures and are representative of two independent experiments. ∗∗p < 0.01, ∗∗∗p < 0.001.