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. 2021 Mar 12;12:601298. doi: 10.3389/fimmu.2021.601298

Figure 3.

Figure 3

PLHIV on ART exhibit CD4 T cell dysregulation and apoptosis due to aberrant DDR. T cell activation, exhaustion, cytokine production, apoptosis, and DNA damage marker expressions in TCR-stimulated or non-stimulated CD4 T cells isolated from HS or PLHIV were determined by Flow Cytometry and Western Blot. (A) Frequency of CD69+ (n = 11) and MFI of pAKT+ cells (n = 11). (B) Representative dot plots and summary data of PD-1+ cells (n = 12). (C) Representative dot plots and summary data of IL-2 expression (n = 12), expression of TGFβ1 (n = 8). (D) Representative histogram and summary MFI of γH2AX in CD4 T cells from HS (n = 18) and PLHIV (n = 21). (E) Expression of active caspase-3 (n = 10), representative dot plots and summary data of Av+7AAD+ CD4 T cells from HS (n = 21) and PLHIV (n = 25), expression of BCL-2 in CD4 T cells from HS and PLHIV (n = 8). (F) Representative imaging and summary data of immunoblotting for PARP-1, pAKT, and γH2AX, normalized by GAPDH.