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. 2021 Mar 12;12:601298. doi: 10.3389/fimmu.2021.601298

Figure 4.

Figure 4

MiR-21 controls CD4 T cell response and apoptosis via regulating the TCR signaling pathways in PLHIV. T cell activation, exhaustion, cytokine production, apoptosis, and DNA damage marker expressions after miR-21 knockdown in CD4 T cells from PLHIV were determined by Flow Cytometry and Western Blot. (A) Relative expression of miR-21 and GAS5 following miR-21 knockdown in CD4 T cells from PLHIV determined by RT-qPCR (n = 5). (B) Frequencies of pMAPK (n = 9), pAKT (n = 9), PD-1 (n = 11), and Tim-3 (n = 8). (C) Representative dot plot and summary data of IL-2 (n = 16), IFN-γ (n = 16), TGFβ1 (n = 8), and TCF1 (n = 7) expressions. (D) Representative dot plot and summary data of Av+ 7AAD+ CD4 T cells (n = 13), expression of active caspase-3 (n = 10). (E) Representative histogram and summary data of γH2AX expression (n = 12). (F) Representative western blots and summary data of cleaved PARP-1 (n = 8), pS6 (n = 6), and γH2AX (n = 6) expressions, normalized to GAPDH.