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. 2021 Mar 12;12:601298. doi: 10.3389/fimmu.2021.601298

Figure 6.

Figure 6

GAS5 controls CD4 T cell response and apoptosis via regulating miR-21-mediated signaling in PLHIV. (A,B) Frequencies of pAKT (n = 11), pMAPK (n = 7), PD-1 (n = 10), and Tim-3 (n = 11) in control vector or GAS5-transducted CD4 T cells from PLHIV, determined by flow cytometry. (C,D) Representative dot plot and summary data of IL-2 expression (n = 10), IFN-γ (n = 12), and TNF-α (n = 5), measured by flow cytometry. (E) Representative histogram and summary data of γH2AX expression (n = 9). (F) Representative dot plot and summary data of Av+7AAD+ CD4 T cells (n = 10) and active caspase-3 (n = 11). (G) Representative western blot imaging and summary data of cleaved PARP-1 (n = 6), pS6 (n = 4), and γH2AX (n = 6) expressions, normalized to GAPDH.