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. 2021 Mar 25;4:407. doi: 10.1038/s42003-021-01939-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Confocal overview of mossy fibers in a Thy-1 mEGFP(Ls1) mouse with imaging window (25 × 25 µm) depicted to scale. b 3D volume reconstruction of mEGFP in xyz-view, showing axons and mossy fiber boutons (green), a typical bouton with filopodial extensions is marked in yellow (black arrow). c 3D reconstruction of Bassoon (magenta) and mEGFP (green) in large mossy fiber boutons used for calculations of “clusters in boutons” in d–i; inset depicts the marked bouton (black arrow) with Bassoon clusters (colored). Cumulative plots of localization counts (d), cluster length (e), and volumes (f) comparing all clusters per image (magenta) and those in identified boutons (blue). Corresponding data as box plots g–i. Scale bar: 100 µm (a), grid size 2 µm (b, c) and scale bar for inset in c 1 µm. Asterisks (***p < 0.001) denote statistical significance between the groups.