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HSCCC chromatogram of 40% methanol fraction using n-hexane–ethyl acetate–methanol–water (3:5:3:5, v/v) (1.5% acetic acid). Conditions: stationary phase, upper phase; revolution speed, 800 rpm; separation temperature, 30 °C; detection wavelength, 320 nm; flow rate, 2.0 ml/min (A: Single injection; B: forth continuous sample injection).
