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HPLC chromatogram of 40% methanol fraction from MCI column. Conditions: column, Platisil ODS-C18 analytical column (250 mm × 4.6 mm i.d., 5 μm); mobile phase, 0.1% acid water (A) and methanol (B), gradient elution program: 0–30 min, 10–95% B; 30–40 min, 95% B; flow rate, 1.0 mL/min; temperature, 30 °C; detection wavelength, 254 nm.
