Fig. 4. Apico-basal cell compression regulates LamC in epithelial tissues.
a Schematic showing the local reduction in cell height by overexpression of a dominant-negative form of CDC42 using Dpp-Gal4. Left panel shows the top-view of the wing disc and the right panel shows the cross-section view of the wing disc. b Cross-section through the DV boundary of the wing disc expressing CDC42F89 by Dpp-Gal4 along the AP boundary. Upper panel shows actin (magenta) and lower panel shows merge between LamC and DAPI. Solid yellow line shows the apical surface, and the blue line shows the basal surface of the cells. Scale bar along both axes, 25 µm. c, d Color-coded LamC images of the regions shown in (b). The pouch region is shown by the light orange ROI and single asterisk, and the ectopic fold (E-fold) region is shown by the dark-orange ROI and double asterisk. Scale bar, 5 µm. e Box-plot showing Dcell in the wing pouch and E-fold. f Box-plot showing normalized LamC in pouch and the E-fold. Normalization is done with respect to pouch. The sample number, n represents the number of wing discs analyzed. g Schematic showing the increase in cell height of PM cells by ectopic overexpression of UAS-Lin using Ubx-Gal4. Left panel shows the top-view of the wing disc and the right panel shows the cross-section view of the wing disc. h Cross-section of the wt wing disc in the region of the wing disc shown by the black ROI. Left panel shows merge of actin (magenta) and Lam C (green). Solid yellow line shows the PM. Right panel shows the color-coded image of LamC. Scale bar along both axes, 25 µm. i Top-view color-coded LamC intensity of the PM (marked by the dark-red ROI and single asterisk) and pouch (light-orange ROI and double asterisk). Scale bar, 5 µm. j Cross-section images of wing disc with ectopic overexpression of UAS-Lin in the region shown by black ROI. Left panel shows merge of actin (magenta) and Lam C (green). Solid yellow line shows the PM. Right panel shows the color-coded image of LamC. Scale bar along both axes, 25 µm. k Top-view color-coded LamC intensity of the PM (marked by the dark-red ROI and single asterisk) and pouch (light-orange ROI and double asterisk). Scale bar, 5 µm. l Box-plot showing Dcell for PM in wt and upon UAS-Lin overexpression. m Box-plot showing normalized LamC for PM in wt and upon UAS-Lin overexpression. The sample number n represents the number of wing discs analyzed. The p values are estimated using a two-sided Student’s t-test and the corresponding p values are given in plots. In the box-plot, horizontal line represents the median of the data, lower and upper bounds of the box represent the 25th and 75th percentile of data, and the whiskers represent the minimum and maximum of the data. The scattered point on the box represents the actual data points.